Product nameAnti-Synaptogyrin 4 antibody
See all Synaptogyrin 4 primary antibodies
DescriptionGoat polyclonal to Synaptogyrin 4
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
- Human brain lysate for Western blot.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab27480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.01 - 01 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa). A minor band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide. Can be blocked with Synaptogyrin 4 peptide (2-14) (ab45658).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Sequence similaritiesBelongs to the synaptogyrin family.
Contains 1 MARVEL domain.
- Information by UniProt
- MGC125805 antibody
- SNG4_HUMAN antibody
- Synaptogyrin-4 antibody
- SYNGR4 antibody
Anti-Synaptogyrin 4 antibody (ab27480) at 0.01 µg/ml + Human brain lysate (35µg protein in RIPA buffer).
Predicted band size: 26 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence. A minor band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunising peptide.
ab27480 has not yet been referenced specifically in any publications.