Anti-Synaptophysin antibody (ab14692)
Key features and details
- Rabbit polyclonal to Synaptophysin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
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Overview
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Product name
Anti-Synaptophysin antibody
See all Synaptophysin primary antibodies -
Description
Rabbit polyclonal to Synaptophysin -
Host species
Rabbit -
Specificity
The antibody does not cross-react other synaptic vesicle proteins such as synaptotagmin, GAP45, and SNAP25 protiens. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Synaptophysin aa 41-62.
Sequence:FATCGSYSGELQLSVDCANKTE
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Positive control
- IHC: Rat and mouse brain. WB: Mouse forebrain extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
The buffer includes stabilizing proteins and glycerol. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa).
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IHC-P | (2) |
1/100.
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Notes |
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WB
1/200 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/100. |
Target
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Function
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
Tissue specificity
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
Involvement in disease
Mental retardation, X-linked, SYP-related -
Sequence similarities
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
Domain
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 20977 Mouse
- Entrez Gene: 24804 Rat
- SwissProt: Q62277 Mouse
- SwissProt: P07825 Rat
- Unigene: 223674 Mouse
- Unigene: 11067 Rat
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Alternative names
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
Images
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Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded mouse brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.
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Synaptophysin antibody (ab14692) western blot on mouse forebrain extract (10µg protein/lane). The blot was exposed for 10 minutes to autoradiographic film. Ladder markers from 7-194 kDa.
Lane 1 = 1/500 ab14692
Lane 2 = 1/200 ab14692 -
Immunohistochemical analysis of formaldehyde-fixed, paraffin-embedded rat brain sections labelling Synaptophysin with ab14692 at a dilution of 1/100 In IHC blocking Buffer. DAB (brown_ staining and Hematoxylin QS (blue) counterstain was used. 20x magnfication of Leica DM4000.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (102)
ab14692 has been referenced in 102 publications.
- Mason MRJ et al. The Jun-dependent axon regeneration gene program: Jun promotes regeneration over plasticity. Hum Mol Genet 31:1242-1262 (2022). PubMed: 34718572
- Xu Y et al. Ketogenic diet ameliorates cognitive impairment and neuroinflammation in a mouse model of Alzheimer's disease. CNS Neurosci Ther 28:580-592 (2022). PubMed: 34889516
- Scaramuzzino C et al. Calcineurin and huntingtin form a calcium-sensing machinery that directs neurotrophic signals to the nucleus. Sci Adv 8:eabj8812 (2022). PubMed: 34985962
- Dentoni G et al. Mitochondria-Endoplasmic Reticulum Interplay Regulates Exo-Cytosis in Human Neuroblastoma Cells. Cells 11:N/A (2022). PubMed: 35159324
- Xie Z et al. Microglial cathepsin E plays a role in neuroinflammation and amyloid β production in Alzheimer's disease. Aging Cell 21:e13565 (2022). PubMed: 35181976