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    synaptophysin-antibody-ab32594.pdf

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Neuroscience Neurotransmission Secretory Vesicles SNAPs & SNAREs
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Anti-Synaptophysin antibody (ab32594)

  • Datasheet
  • SDS
Reviews (13)Q&A (4)References (42)

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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
  • Western blot - Anti-Synaptophysin antibody (ab32594)
  • Western blot - Anti-Synaptophysin antibody (ab32594)
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
  • Western blot - Anti-Synaptophysin antibody (ab32594)

Key features and details

  • Rabbit polyclonal to Synaptophysin
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Synaptophysin antibody [EPR1097-2] (ab178412)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Synaptophysin antibody
    See all Synaptophysin primary antibodies
  • Description

    Rabbit polyclonal to Synaptophysin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide:

    GPGGYGPQDSYGPQGGYQPD

    , corresponding to amino acids 253-272 of Rat Synaptophysin
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse and rat brain tissue lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human Synaptophysin protein (ab114166)
  • Recombinant Protein

    • Recombinant Human Synaptophysin protein (ab114166)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (5)
1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa).
ICC/IF (1)
Use at an assay dependent concentration.
Notes
WB
1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa).
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificity

    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in disease

    Mental retardation, X-linked, SYP-related
  • Sequence similarities

    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • Domain

    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Target information above from: UniProt accession P08247 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6855 Human
    • Entrez Gene: 24804 Rat
    • Omim: 313475 Human
    • SwissProt: P08247 Human
    • SwissProt: P07825 Rat
    • Unigene: 632804 Human
    • Unigene: 11067 Rat
    • Alternative names

      • Major synaptic vesicle protein p38 antibody
      • MRX96 antibody
      • MRXSYP antibody
      • Syn p38 antibody
      • Synaptophysin antibody
      • Syp antibody
      • SYPH antibody
      • SYPH_HUMAN antibody
      • SypI antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
      Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

      ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

    • Western blot - Anti-Synaptophysin antibody (ab32594)
      Western blot - Anti-Synaptophysin antibody (ab32594)
      All lanes : Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate
      Lane 3 : Rat liver tissue lysate
      Lane 4 : Rat kidney tissue lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 34 kDa



      Western blot analysis was performed on tissue extracts (30 µg lysate). The blot was probed with ab32594 (1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate. A 34 kDa band corresponding to Synaptophysin was observed in Mouse Brain, Rat Brain and not observed in other tissues which are documented to be Synaptophysin negative.

    • Western blot - Anti-Synaptophysin antibody (ab32594)
      Western blot - Anti-Synaptophysin antibody (ab32594)This image is courtesy of an anonymous Abreview
      Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg

      Secondary
      AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG
      at 1/1000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 33,35 kDa why is the actual band size different from the predicted?


      Exposure time: 7 minutes

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
      Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

      ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

    • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
      Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

      ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

    • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
      Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

      ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

    • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
      Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

      ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

    • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
      Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

      ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

    • Western blot - Anti-Synaptophysin antibody (ab32594)
      Western blot - Anti-Synaptophysin antibody (ab32594)
      Anti-Synaptophysin antibody (ab32594) at 1/5000 dilution + Rat brain

      Predicted band size: 34 kDa
      Observed band size: 33.8 kDa why is the actual band size different from the predicted?

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (42)

    Publishing research using ab32594? Please let us know so that we can cite the reference in this datasheet.

    ab32594 has been referenced in 42 publications.

    • Kushwaha R  et al. Non-cell autonomous astrocyte-mediated neuronal toxicity in prion diseases. Acta Neuropathol Commun 9:22 (2021). PubMed: 33546775
    • Ingwersen T  et al. G392E neuroserpin causing the dementia FENIB is secreted from cells but is not synaptotoxic. Sci Rep 11:8766 (2021). PubMed: 33888787
    • Joviano-Santos JV  et al. Motoneuron-specific loss of VAChT mimics neuromuscular defects seen in congenital myasthenic syndrome. FEBS J N/A:N/A (2021). PubMed: 33730374
    • Grinman E  et al. Activity-regulated synaptic targeting of lncRNA ADEPTR mediates structural plasticity by localizing Sptn1 and AnkB in dendrites. Sci Adv 7:N/A (2021). PubMed: 33863727
    • Sinha A  et al. Phagocytic Activities of Reactive Microglia and Astrocytes Associated with Prion Diseases Are Dysregulated in Opposite Directions. Cells 10:N/A (2021). PubMed: 34359897
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-Synaptophysin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Cerebellum)
    Gel Running Conditions
    Reduced Denaturing (4-15% gradient)
    Loading amount
    20 µg
    Specification
    Cerebellum
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 02 2021

    Western blot abreview for Anti-Synaptophysin antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Whole brain tissue lysate)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (3-8% Tris-Acetate)
    Loading amount
    20 µg
    Specification
    Whole brain tissue lysate
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 05 2017

    Western blot abreview for Anti-Synaptophysin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (Brain)
    Loading amount
    10 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ayça Arslan Ergül

    Verified customer

    Submitted Dec 31 2012

    Western blot abreview for Anti-Synaptophysin antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount
    50 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 26 2012

    Western blot abreview for Anti-Synaptophysin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Zebrafish Tissue lysate - whole (brain)
    Loading amount
    21 µg
    Specification
    brain
    Gel Running Conditions
    Reduced Denaturing (10% resolving SDS-PAGE gel)
    Blocking step
    Milk as blocking agent for 48 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mrs. Fusun Doldur Balli

    Verified customer

    Submitted Apr 12 2012

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