Anti-Synaptophysin antibody (ab32594)
Key features and details
- Rabbit polyclonal to Synaptophysin
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-Synaptophysin antibody
See all Synaptophysin primary antibodies -
Description
Rabbit polyclonal to Synaptophysin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Human
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Immunogen
Synthetic peptide:
GPGGYGPQDSYGPQGGYQPD
, corresponding to amino acids 253-272 of Rat Synaptophysin -
Positive control
- WB: Mouse and rat brain tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa).
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa). |
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ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
Tissue specificity
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
Involvement in disease
Mental retardation, X-linked, SYP-related -
Sequence similarities
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
Domain
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 6855 Human
- Entrez Gene: 24804 Rat
- Omim: 313475 Human
- SwissProt: P08247 Human
- SwissProt: P07825 Rat
- Unigene: 632804 Human
- Unigene: 11067 Rat
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Alternative names
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Western blot - Anti-Synaptophysin antibody (ab32594)All lanes : Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat liver tissue lysate
Lane 4 : Rat kidney tissue lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDaWestern blot analysis was performed on tissue extracts (30 µg lysate). The blot was probed with ab32594 (1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate. A 34 kDa band corresponding to Synaptophysin was observed in Mouse Brain, Rat Brain and not observed in other tissues which are documented to be Synaptophysin negative.
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Western blot - Anti-Synaptophysin antibody (ab32594)This image is courtesy of an anonymous AbreviewAnti-Synaptophysin antibody (ab32594) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg
Secondary
AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG
at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 33,35 kDa why is the actual band size different from the predicted?
Exposure time: 7 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Western blot - Anti-Synaptophysin antibody (ab32594)Anti-Synaptophysin antibody (ab32594) at 1/5000 dilution + Rat brain
Predicted band size: 34 kDa
Observed band size: 33.8 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (42)
ab32594 has been referenced in 42 publications.
- Kushwaha R et al. Non-cell autonomous astrocyte-mediated neuronal toxicity in prion diseases. Acta Neuropathol Commun 9:22 (2021). PubMed: 33546775
- Ingwersen T et al. G392E neuroserpin causing the dementia FENIB is secreted from cells but is not synaptotoxic. Sci Rep 11:8766 (2021). PubMed: 33888787
- Joviano-Santos JV et al. Motoneuron-specific loss of VAChT mimics neuromuscular defects seen in congenital myasthenic syndrome. FEBS J N/A:N/A (2021). PubMed: 33730374
- Grinman E et al. Activity-regulated synaptic targeting of lncRNA ADEPTR mediates structural plasticity by localizing Sptn1 and AnkB in dendrites. Sci Adv 7:N/A (2021). PubMed: 33863727
- Sinha A et al. Phagocytic Activities of Reactive Microglia and Astrocytes Associated with Prion Diseases Are Dysregulated in Opposite Directions. Cells 10:N/A (2021). PubMed: 34359897