Anti-Synaptophysin antibody (ab32594)

Rabbit polyclonal Synaptophysin antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Sheep, Cow, Zebrafish, Apteronotus leptorhynchus. Cited in 14 publication(s).

Reviews (8)Q&A (4)References (19)

Overview

  • Product name

    Anti-Synaptophysin antibody
    See all Synaptophysin primary antibodies

  • Description

    Rabbit polyclonal to Synaptophysin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-FoFr, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Cow, Zebrafish, Apteronotus leptorhynchus
    Predicted to work with: Human

  • Immunogen

    Synthetic peptide:

    GPGGYGPQDSYGPQGGYQPD

    , corresponding to amino acids 253-272 of Rat Synaptophysin

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant Human Synaptophysin protein (ab114166) can be used as a positive control in WB. Rat, mouse and bovine brain tissue and sheep retinas.

Properties

Applications

Our Abpromise guarantee covers the use of ab32594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa).
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.

  • Tissue specificity

    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

  • Involvement in disease

    Mental retardation, X-linked, SYP-related

  • Sequence similarities

    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.

  • Domain

    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.

  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Images

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Synaptophysin antibody (ab32594)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Synaptophysin antibody (ab32594)This image is courtesy of an anonymous Abreview
    Immunohistochemical analysis of Apteronotus leptorhynchus brain tissue, staining Synaptophysin with ab32594.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% sheep serum, 1% BSA and 1% teleostean gelatine in TBS for 1 hour at 24°. Samples were incubated with primary antibody (1/100 in blocking solution) for 18 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

    ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • Western blot - Anti-Synaptophysin antibody (ab32594)
    Western blot - Anti-Synaptophysin antibody (ab32594)This image is courtesy of an anonymous Abreview
    Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg

    Secondary
    AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG
    at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 33,35 kDa
    why is the actual band size different from the predicted?


    Exposure time: 7 minutes

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

    ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

    ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

    ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

    ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)

    ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.

  • Western blot - Anti-Synaptophysin antibody (ab32594)
    Western blot - Anti-Synaptophysin antibody (ab32594)
    Anti-Synaptophysin antibody (ab32594) at 1/5000 dilution + Rat brain

    Predicted band size: 34 kDa
    Observed band size: 33.8 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Matsui H  et al. Age- and a-Synuclein-Dependent Degeneration of Dopamine and Noradrenaline Neurons in the Annual Killifish Nothobranchius furzeri. Cell Rep 26:1727-1733.e6 (2019). Read more (PubMed: 30759385) »
  • Maryanovich M  et al. Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche. Nat Med 24:782-791 (2018). Read more (PubMed: 29736022) »
See all 19 Publications for this product

Publishing research using ab32594? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 12 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Synaptophysin antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Xenopus laevis Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - Triton
Specification
Spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Oct 09 2018

Western blot abreview for Anti-Synaptophysin antibody

 Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-8% Tris-Acetate)
Loading amount
20 µg
Specification
Whole brain tissue lysate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Jun 05 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Synaptophysin antibody

 Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Cell (hippocampal nueron)
Specification
hippocampal nueron
Permeabilization
Yes - triton x-100
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 13 2014

Immunohistochemistry (Frozen sections) abreview for Anti-Synaptophysin antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Sample
Mouse Tissue sections (Skeletal muscle)
Specification
Skeletal muscle
Permeabilization
Yes - 1% Triton X-100
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 08 2014

Western blot abreview for Anti-Synaptophysin antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Brain)
Loading amount
10 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. Ayça Arslan Ergül

Verified customer

Submitted Dec 31 2012

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Synaptophysin antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Nov 30 2012

Western blot abreview for Anti-Synaptophysin antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Nov 26 2012

Western blot abreview for Anti-Synaptophysin antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (brain)
Loading amount
21 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10% resolving SDS-PAGE gel)
Blocking step
Milk as blocking agent for 48 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Mrs. Fusun Doldur Balli

Verified customer

Submitted Apr 12 2012

Question


Thank you for your prompt reply. I have submitted our Abreviews for 3
primary antibodies. However I am now aware of not having included testing
codes to our Abreviews.
I have attached more detailed reports of our lab to this e-mail regarding
our western blot experiments. Results with anti Actin and anti
Synaptophysin primary antibodies are fine but results with anti PSD-95
primary antibody are inconclusive for Zebrafish brain lysates. Can you
offer us another anti PSD-95 primary antibody among your companies
products? One more question, is it possible for you to give a code for the
new anti PSD-95 antibody so that we may report whether it reacts with
Zebrafish proteins or not. And if it does not work either can we have a
right to try another antibody for free for third trial?
Thanks in advance,

Read More
Answer

Thank you for contacting me and letting me know about this situation.

I have entered your Abreview codes into the corresponding reviews, so these are now active and can be redeemed for free primary antibodies.

I am sorry to see that the results with the PSD-95 antibody were inconclusive. You can use one ofthe testing codes to get a different PSD-95 antibody for freeif you would like, and ab90426 would be my recommendation to try next. In order to provide a code to test an antibody, you will need to purchase the test antibody at full price, so I can not issue a code for ab90426 if you will be using one of the older codes to get ab90426 for free. So, I can either issue a code for ab90426 and you can purchase this at full price, or you can use one of your older codes to acquire ab90426 for free. Please let me know which option you would like, or if you have any questions about this process.

I hope this will be helpful, but please let me know if there is anything else that we can do for you.

Read More

Question

Hello,
We have tried troubleshooting suggestions from Abcam about antibodies and
we want to submit Abreviews. Can you please explain how to submit an
Abreview?
Thanks in advance,

Read More
Answer

Thank you for your reply.

You can submit an Abreview by logging into your Abcam account and clicking on "Abreviews" on the left side of the screen underneath "My Account". Then, click on "Write an Abreview" and select the options for your review.

You can also submit a review from the datasheet of each antibody. On the datasheet for the antibody you have used, go to the "Abreview" tab and then follow the link to "Submit an Abreview". Then all you need to do is select the application and sample species.

After that you simply need to work through the form, and make sure to include each testing code in the "Additional Notes" section of each review. Once the code is added to the review, it will be active and can be submitted with your order through Kimera for a free antibody per code.

I hope this information will be useful, but please let me know if you have any further questions or need anything else, and I'll be happy to help.

Read More

1-10 of 12 Abreviews or Q&A

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