Anti-Synaptophysin antibody (ab32594)
- Datasheet
- References (13)
- Protocols
Overview
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Product nameAnti-Synaptophysin antibody
See all Synaptophysin primary antibodies -
DescriptionRabbit polyclonal to Synaptophysin
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-FoFr, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Sheep, Cow, Zebrafish, Apteronotus leptorhynchus
Predicted to work with: Human
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Immunogen
Synthetic peptide:
GPGGYGPQDSYGPQGGYQPD
, corresponding to amino acids 253-272 of Rat Synaptophysin -
Positive control
- Recombinant Human Synaptophysin protein (ab114166) can be used as a positive control in WB. Rat, mouse and bovine brain tissue and sheep retinas.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab32594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 33.8 kDa (predicted molecular weight: 34 kDa). | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
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Tissue specificityCharacteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
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Involvement in diseaseMental retardation, X-linked, SYP-related
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Sequence similaritiesBelongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
DomainThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
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Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
- Information by UniProt
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Database links
- Entrez Gene: 280937 Cow
- Entrez Gene: 6855 Human
- Entrez Gene: 20977 Mouse
- Entrez Gene: 24804 Rat
- Entrez Gene: 559698 Zebrafish
- Omim: 313475 Human
- SwissProt: P20488 Cow
- SwissProt: P08247 Human
see all -
Alternative names
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Western blot - Anti-Synaptophysin antibody (ab32594)This image is courtesy of an anonymous AbreviewAnti-Synaptophysin antibody (ab32594) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg
Secondary
AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG
at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 33,35 kDa why is the actual band size different from the predicted?
Exposure time: 7 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody (ab32594)ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Western blot - Anti-Synaptophysin antibody (ab32594)Anti-Synaptophysin antibody (ab32594) at 1/5000 dilution + Rat brain
Predicted band size: 34 kDa
Observed band size: 33.8 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Synaptophysin antibody (ab32594)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of Apteronotus leptorhynchus brain tissue, staining Synaptophysin with ab32594.
Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 3% sheep serum, 1% BSA and 1% teleostean gelatine in TBS for 1 hour at 24°. Samples were incubated with primary antibody (1/100 in blocking solution) for 18 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Chia K et al. Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain. Elife 7:N/A (2018). Read more (PubMed: 29465400) »
- Liu CX et al. CRISPR/Cas9-induced shank3b mutant zebrafish display autism-like behaviors. Mol Autism 9:23 (2018). Read more (PubMed: 29619162) »
