Anti-Synaptophysin antibody [SY38] (ab8049)

Reviews (12)Q&A (11)References (99)

Overview

  • Product name

    Anti-Synaptophysin antibody [SY38]
    See all Synaptophysin primary antibodies

  • Description

    Mouse monoclonal [SY38] to Synaptophysin

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Hamster, Cow, Human

  • Immunogen

    Presynaptic vesicles.

  • Positive control

    • Adrenal pheochromocytoma and pancreatic islet cell neoplasm

Properties

Applications

Our Abpromise guarantee covers the use of ab8049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
Flow Cyt 1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/10.
IHC-P 1/10.

Target

  • Function

    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.

  • Tissue specificity

    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.

  • Involvement in disease

    Mental retardation, X-linked, SYP-related

  • Sequence similarities

    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.

  • Domain

    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.

  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)This image is courtesy of an Abreview provided by Carl Hobbs

    ab8049 staining Synaptophysin in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/100) for 16 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-Synaptophysin antibody [SY38] (ab8049)
    Western blot - Anti-Synaptophysin antibody [SY38] (ab8049)
    All lanes : Anti-Synaptophysin antibody [SY38] (ab8049) at 1/500 dilution

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution

    Predicted band size: 34 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Frozen sections) - Anti-Synaptophysin antibody [SY38] (ab8049)
    Immunohistochemistry (Frozen sections) - Anti-Synaptophysin antibody [SY38] (ab8049)This image is courtesy of an anonymous Abreview

    ab8049 staining Synaptophysin in mouse hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)This image is courtesy of an Abreview provided by Carl Hobbs.

    ab8049 staining MAFA in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouseIgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [SY38] (ab8049)

    The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine.  The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature,  
    secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.

    These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.

  • Flow Cytometry - Anti-Synaptophysin antibody [SY38] (ab8049)
    Flow Cytometry - Anti-Synaptophysin antibody [SY38] (ab8049)
    Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

References

This product has been referenced in:

  • Naskar A  et al. Aging mildly affects dendritic arborisation and synaptic protein expression in human substantia nigra pars compacta. J Chem Neuroanat 97:57-65 (2019). Read more (PubMed: 30738909) »
  • van de Looij Y  et al. Nutritional Intervention for Developmental Brain Damage: Effects of Lactoferrin Supplementation in Hypocaloric Induced Intrauterine Growth Restriction Rat Pups. Front Endocrinol (Lausanne) 10:46 (2019). Read more (PubMed: 30800096) »
See all 99 Publications for this product

Publishing research using ab8049? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 23 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synaptophysin antibody [SY38]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted May 16 2016

IHC - Wholemount abreview for Anti-Synaptophysin antibody [SY38]

 Excellent
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (Skeletal muscle)
Specification
Skeletal muscle
Read More

Abcam user community

Verified customer

Submitted Apr 08 2016

Immunocytochemistry abreview for Anti-Synaptophysin antibody [SY38]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (cortical neurons primary culture)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
cortical neurons primary culture
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 01 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synaptophysin antibody [SY38]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More

Mr. Carl Hobbs

Verified customer

Submitted Dec 22 2015

Flow Cytometry abreview for Anti-Synaptophysin antibody [SY38]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Aug 15 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Synaptophysin antibody [SY38]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (Differentiated hNSCs)
Specification
Differentiated hNSCs
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Feb 16 2015

Immunohistochemistry (Frozen sections) abreview for Anti-Synaptophysin antibody [SY38]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Mouse Tissue sections (hippocampus)
Specification
hippocampus
Permeabilization
No
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 20 2015

Question


We have not had much success finding a synaptic marker that works on paraffin-embedded human brain tissue. I did not see a specific reference for this antibody or an image from human brain. Would you be able to provide this, so that we are better able to decide whether this antibody will work for our studies?

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Answer

Thanks for your interest in ab8049.

All the information we have regarding this antibody is present on the online product datasheet at https://www.abcam.com/synaptophysin-antibody-sy38-ab8049.html. This includes customer-submitted reviews (Abreviews https://www.abcam.com/Synaptophysin-antibody-SY38-ab8049-reviews.html) and all references we know of where this antiody has been used (Specific references https://www.abcam.com/Synaptophysin-antibody-SY38-ab8049-references.html).

I hope this is helpful. Please contact us again if you ha ve any further questions.

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Question

I would like to enroll in your AbTrial Program. I will be using your antibodies for immunohistochemical experiments with Rhesus monkey brain tissue.

We will be examining synaptic markers inbrain tissue of rhesus macaques previously exposed to SIV.

Since your antibodies do not say that they work in rhesus monkey, I am requesting a trial for the testing of the following antibodies:

anti-synaptophysin (ab8049)

anti-hypophosphorylated neurofilament (ab82259)

antisynapsin (ab64581)

anti-PSD95 (ab18258)

anti-cathepsin B (ab58802)

anti-IBA1 (ab5076)

anti-GFAP (ab4674)

I have just placed a purchase order for these antibodies.

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Answer

I am very pleased to hear you would like to test ab5076, ab8049, ab18258, ab58802, ab64581, ab82259 and send us your results. The testing discount doesn't apply to ab4674 since this has already been tested in Rhesus monkey and IHC so we would guarantee that experiment to work.

The codes will give you a value off your next orders before the expiration date. To redeem this offer, please submit 6 Abreview with rhesus monkey data and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview wit has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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Question

hello I wish to purchase ab8049 mouse monoclonal anti-synaptophysin. It says that the recommended conc for IHC-P is 1:10. This is prediluted stock? and what is the exact concentration of this stock? thank you

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Answer

Thank you for contacting us. This antibody is from ascites and is not a purified antibody. Generally concentrations are only measured for purified antibodies; they are not relevant for antibodies from ascites or whole serum.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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