Overview

  • Product name

    Anti-Synaptophysin antibody [SY38]
    See all Synaptophysin primary antibodies
  • Description

    Mouse monoclonal [SY38] to Synaptophysin
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Hamster, Cow, Human
  • Immunogen

    Presynaptic vesicles.

  • Positive control

    • Adrenal pheochromocytoma and pancreatic islet cell neoplasm

Properties

Applications

Our Abpromise guarantee covers the use of ab8049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
Flow Cyt 1/20.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/10.
IHC-P 1/10.

Target

  • Function

    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificity

    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in disease

    Mental retardation, X-linked, SYP-related
  • Sequence similarities

    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • Domain

    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Images

  • ab8049 staining Synaptophysin in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (1/100) for 16 hours at 21°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Synaptophysin antibody [SY38] (ab8049) at 1/500 dilution

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed at 1/3000 dilution

    Predicted band size: 34 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • ab8049 staining Synaptophysin in mouse hippocampus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in BSA) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab8049 staining MAFA in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/100 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouseIgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • The pictures show immunohistochemical staining of normal mucosa and neuroendocrine tumour of the small intestine.  The antibody (ab8049) stains positive in a 1:10 dilution in nerve cells of the colon as well as some cells in normal colon mucosa and some neuroendocrine tumors of the digestive tract. Staining protocol: Mouse monoclonal to Synaptophysin (ab8049), diluted 1:10 in PBS plus BSA, 60 min at room temperature,  
    secondary antibody: HRP solution with anti mouse polyclonal antibodies, 30min. DAB staining 10 min; Hematoxylin 30 sec.

    These images were kindly supplied as part of the review submitted by Karin Birkenkamp-Demtroeder.

  • Overlay histogram showing PC12 cells stained with ab8049 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8049, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

References

This product has been referenced in:

  • Naskar A  et al. Aging mildly affects dendritic arborisation and synaptic protein expression in human substantia nigra pars compacta. J Chem Neuroanat 97:57-65 (2019). Read more (PubMed: 30738909) »
  • van de Looij Y  et al. Nutritional Intervention for Developmental Brain Damage: Effects of Lactoferrin Supplementation in Hypocaloric Induced Intrauterine Growth Restriction Rat Pups. Front Endocrinol (Lausanne) 10:46 (2019). Read more (PubMed: 30800096) »
See all 87 Publications for this product

Customer reviews and Q&As

1-10 of 11 Q&A

Answer

Thanks for your interest in ab8049.

All the information we have regarding this antibody is present on the online product datasheet at https://www.abcam.com/synaptophysin-antibody-sy38-ab8049.html. This includes customer-submitted reviews (Abreviews https://www.abcam.com/Synaptophysin-antibody-SY38-ab8049-reviews.html) and all references we know of where this antiody has been used (Specific references https://www.abcam.com/Synaptophysin-antibody-SY38-ab8049-references.html).

I hope this is helpful. Please contact us again if you ha ve any further questions.

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Answer

I am very pleased to hear you would like to test ab5076, ab8049, ab18258, ab58802, ab64581, ab82259 and send us your results. The testing discount doesn't apply to ab4674 since this has already been tested in Rhesus monkey and IHC so we would guarantee that experiment to work.

The codes will give you a value off your next orders before the expiration date. To redeem this offer, please submit 6 Abreview with rhesus monkey data and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview wit has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

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Answer

Thank you for contacting us. This antibody is from ascites and is not a purified antibody. Generally concentrations are only measured for purified antibodies; they are not relevant for antibodies from ascites or whole serum.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456 und solltemorgen bei Ihnen ankommen.

Des Weiteren finden Sie noch unseren Fragebogen im Anhang, damit wir dem Problem auf den Grund gehen koennen.

Ich wünsche Ihnen viel Erfolg für Ihr Projekt. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question
Answer

Thank you for contacting us.

Here please find the information about our testing discount program. Below I also include information about our Abpromise guarantee.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.


Abpromise guarantee:

We guarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for calling Abcam. The immunogen for ab8049 is the vesicular fraction of bovine brain and the epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus & Betz 1990). Unfortunately the exact immunogen sequence is not known, and we are at this time unable to provide the peptide for ab8049. Based on this information I do not think that any of the synaptophysin peptides in our catalogue would be successful in blocking the activity of ab8049. There is always a chance though. If there is anything else I can help you with, please let me know.  

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Answer

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab8049 in IHC. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. Can you confirm that the second antibody you used also managed to produce good results with other primary antibodies? In some cases, the problem may be with the secondary antibody not working properly. Did you get any background staining when you run a “no primary” control? Most formalin-fixed tissue requires an antigen retrieval step before immunohistochemical staining can proceed. This is due to the formation of methylene bridges during fixation, which cross-link proteins and therefore mask antigenic sites. Antigen retrieval with Tris/EDTA pH 9.0 buffer is suitable for most antigens. Sodium citrate pH 6.0 is also widely used. Please use one of these buffers instead of deionised water. I am afraid that you are using this primary antibody (mouse host species) with mouse samples. This means that your secondary antibody (anti-mouse IgG) will bind to all the endogenous IgG in the mouse tissue and high non-specific background would occur. There are a few suggestions we can make to try and improve your results when doing mouse-on-mouse staining. However, please note that your success may vary and we cannot guarantee that you will see a reduction in non-specific staining. IHCWorld.com describes an additional blocking step using unconjugated fab fragment anti-mouse IgG to block endogenous IgG present in the tissue sample. Please see the following link for further information: http://ihcworld.com/_protocols/general_IHC/immuno_mom.htm In addition, kits such as Dako’s "Animal Research kit" (ARK) allows the direct biotinylation of the primary Ab being used, negating the necessity for a secondary Ab and reducing the potential for non specific staining. The following paper (Van der Loos et al 2000) gives a summary of the use of these types of kit: http://www.jhc.org/cgi/content/full/48/10/1431 I hope the above recommendations may already help you. If you have already tried the above suggestions and still experience problems, please do not hesitate to contact me again. Also, please advice me on how you would like to proceed with your enquiry, so that I can immediately arrange for a replacement or refund to you.

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Answer

Thank you for your enquiry and your interest in our products. We would suggest fixing the samples either in ice-cold acetone (for 5-10 min), methanol or ethanol.

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Answer

Thank you for your e-mail. I'm sorry to hear you are having problems with this antibody and would like to help. Thank you very much for providing a photograph of the staining, it is very useful. I would like to ask a few questions and suggest some modifications: -The datasheet says: proteolytic or T.U.F. pretreatment is not necessary but may, depending on the length of the fixation period, enhance the staining. Have you tried this pretreatment? -Do you do antigen retrieval if these are paraffin embedded formalin fixed sections? -The datasheet also recommends trying a 1:10 dilution. Have you tried this? Have you tried an overnight incubation at 4C? -Do you have triton x100 in your dilution buffers to enable an adequate penetration of the antibody into the tissue? I very much hope the above advice will help you, but if you are still unhappy with this product please e-mail me and I will arranged a replacement or refund.

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Question
Answer

Thank you for your enquiry. The concentration of this product is 10 ug/ml.

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1-10 of 11 Q&A

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