Western blot abreview for Anti-Synaptophysin [SY38] antibody

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Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (neuronal)
Loading amount
30 µg
Specification
neuronal
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
odyssey block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: pbs

Secondary antibody

Name
Non-Abcam antibody was used: goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000

Detection

Detection method
LiCor
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: approx 40 kDa
Positive control
lane 1 - synaptic protein extract
Negative control
lane 2 - cytosol

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Submitted Sep 25 2012

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