Anti-Synaptophysin antibody [YE269] (ab32127)

Blocking/Dilution buffer: 5% NFDM/TBST

Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab32127 at a dilution of 1/400.

A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

ab32127 staining synaptophysin in human iPS cell derived neurons by immunocytochemistry/immmunofluorescence.

Samples were fixed with paraformaldehyde and blocked with 1% serum for 30 minutes at room temperature. Samples were incubated with primary antibody at 1/250 dilution for 1 hour. ab150061 was used as the secondary antibody at 1/250 dilution. 

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Immunofluorescent staining of PC-12 (rat adrenal gland pheochromocytoma cell line) cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab32127 at a dilution of 1/50.

An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counterstained with DAPI.

The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor^® 594 goat anti-mouse was used at a dilution of 1/500.

ab32127 staining Synaptophysin in rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% BSA for 2 hours at 25°C. Samples were incubated with primary antibody at 1/300 dilution for 10 hours at 4°C. An Alexa Fluor^® 555 rabbit polyclonal was used as a secondary antibody at 1/400 dilution.

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Unpurified ab32127 showing positive staining in medulloblastoma tissue.

Samples were incubated with primary antibody for 16 hours at 4°C in 5% milk in TBST.

Blocking buffer: 5% milk for 30 minutes at room temperature. 

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Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab32127 at a dilution of 1/400.

A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of paraffin embedded human pancreas with purified ab32127 at a dilution of 1/400.

A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Unpurified ab32127 showing positive staining in lung neuroendocrine tumor tissue.