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  1. Link

    synaptophysin-antibody-ye269-ab32127.pdf

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Neuroscience Neurotransmission Secretory Vesicles SNAPs & SNAREs
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Synaptophysin antibody [YE269] (ab32127)

  • Datasheet
  • SDS
Reviews (37)Q&A (3)References (184)

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Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)
  • Anti-Synaptophysin antibody [YE269] (ab32127)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [YE269] to Synaptophysin
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP

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Anti-beta III Tubulin antibody - Neuronal Marker (ab18207)
Knockout
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Human SYP (Synaptophysin) knockout HEK-293T cell line (ab255356)
Primary
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Anti-MAP2 antibody - Neuronal Marker (ab32454)

View more associated products

Overview

  • Product name

    Anti-Synaptophysin antibody [YE269]
    See all Synaptophysin primary antibodies
  • Description

    Rabbit monoclonal [YE269] to Synaptophysin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Donkey, Cow
  • Immunogen

    Synthetic peptide within Human Synaptophysin aa 250 to the C-terminus (C terminal). The exact sequence is proprietary. Residues in the cytoplasmic domain of human Synaptophysin.
    Database link: P08247
    (Peptide available as ab189853)

  • Positive control

    • WB: PC-12 and HEK-293T cell lysates; Human fetal brain, mouse brain and rat brain lysates; Neurons from iPS cells lysate. ICC/IF: PC-12 cells; Human iPS cell derived neurons, primary mouse neurons/glia, DIV14 cells. IHC-P: Human pancreas, mouse cerebral cortex, rat cerebral cortex, medullablastoma, lung neuroendocrine tumor tissues; Sheep gut tissue. IHC-Fr: Rat brain tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    YE269
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
    • HRP Anti-Synaptophysin antibody [YE269] (ab195520)
    • Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] (ab196166)
    • Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] (ab196379)
    • Alexa Fluor® 594 Anti-Synaptophysin antibody [YE269] (ab206868)
    • Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Immunizing Peptide (Blocking)

    • Synaptophysin peptide (ab189853)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human SYP (Synaptophysin) knockout HEK-293T cell line (ab255356)
    • Human SYP (Synaptophysin) knockout HEK-293T cell line (ab267272)
  • KO cell lysates

    • Human SYP (Synaptophysin) knockout HEK-293T cell lysate (ab257060)
    • Human SYP (Synaptophysin) knockout HEK-293T cell lysate (ab263862)
  • KO cell pellets

    • Human SYP (Synaptophysin) knockout HEK-293T cell pellet (ab278946)
  • Recombinant Protein

    • Recombinant Human Synaptophysin protein (ab114166)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (7)
1/20000 - 1/100000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).Can be blocked with Synaptophysin peptide (ab189853).
IHC-P (13)
1/400 - 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF (3)
Use a concentration of 0.1 µg/ml.
Notes
WB
1/20000 - 1/100000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).Can be blocked with Synaptophysin peptide (ab189853).
IHC-P
1/400 - 1/800. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF
Use a concentration of 0.1 µg/ml.

Target

  • Function

    Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
  • Tissue specificity

    Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • Involvement in disease

    Mental retardation, X-linked, SYP-related
  • Sequence similarities

    Belongs to the synaptophysin/synaptobrevin family.
    Contains 1 MARVEL domain.
  • Domain

    The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization

    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
  • Target information above from: UniProt accession P08247 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 280937 Cow
    • Entrez Gene: 6855 Human
    • Entrez Gene: 20977 Mouse
    • Entrez Gene: 24804 Rat
    • Omim: 313475 Human
    • SwissProt: P20488 Cow
    • SwissProt: P08247 Human
    • SwissProt: Q62277 Mouse
    • SwissProt: P07825 Rat
    • Unigene: 632804 Human
    • Unigene: 223674 Mouse
    • Unigene: 11067 Rat
    see all
  • Alternative names

    • Major synaptic vesicle protein p38 antibody
    • MRX96 antibody
    • MRXSYP antibody
    • Syn p38 antibody
    • Synaptophysin antibody
    • Syp antibody
    • SYPH antibody
    • SYPH_HUMAN antibody
    • SypI antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron cells labeling Synaptophysin with purified ab32127 at 1/100 (2.7µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)

    ab32127 staining Synaptophysin in primary mouse neurons/glia, DIV14 (prepared from E18 mouse hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP) cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab32127 at 0.1µg/ml and ab192757, Mouse mono Anti-PSD95 antibody [K28/43] - Synaptic Marker. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
    All lanes : Anti-Synaptophysin antibody [YE269] (ab32127) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : SYP knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab32127 observed at 38 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab32127 was shown to react with Syp in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab255356 (knockout cell lysate ab263862) was used. Wild-type HEK-293T and SYP knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32127 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)

    ab32127 staining Synaptophysin in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab32127 at 0.1µg/ml and ab192757, Mouse mono Anti-PSD95 antibody [K28/43] - Synaptic Marker. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab32127 at a dilution of 1/400.

    A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
    All lanes : Anti-Synaptophysin antibody [YE269] (ab32127) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate at 20 µg
    Lane 2 : SYP knockout HEK-293T cell lysate at 20 µg
    Lane 3 : Human brain tissue lysate

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab32127 observed at 38 kDa. Red - loading control, ab7291 observed at 50 kDa.

    ab32127 Anti-Synaptophysin antibody [YE269] was shown to specifically react with Synaptophysin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267272 (knockout cell lysate ab257060) was used. Wild-type and Synaptophysin knockout samples were subjected to SDS-PAGE. ab32127 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunofluorescent staining of PC-12 (rat adrenal gland pheochromocytoma cell line) cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab32127 at a dilution of 1/50.

    An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/500 and the cells were counterstained with DAPI.

    The negative control is shown in the bottom right hand panel - for the negative control, Alex Fluor® 594 goat anti-mouse was used at a dilution of 1/500.

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
    All lanes : Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution (purified)

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Secondary
    All lanes : HRP goat anti-rabbit at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Unpurified ab32127 showing positive staining in medulloblastoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab32127 at a dilution of 1/400.

    A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Immunohistochemical staining of paraffin embedded human pancreas with purified ab32127 at a dilution of 1/400.

    A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
    Anti-Synaptophysin antibody [YE269] (ab32127) at 1/100000 dilution (purified) + Human fetal brain at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
    Western blot - Anti-Synaptophysin antibody [YE269] (ab32127)
    Anti-Synaptophysin antibody [YE269] (ab32127) at 1/10000 dilution (unpurified) + PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

    Predicted band size: 34 kDa
    Observed band size: 34 kDa
    Additional bands at: 21 kDa, 22 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269] (ab32127)

    Unpurified ab32127 showing positive staining in lung neuroendocrine tumor tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Synaptophysin antibody [YE269] (ab32127)
    Anti-Synaptophysin antibody [YE269] (ab32127)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (184)

Publishing research using ab32127? Please let us know so that we can cite the reference in this datasheet.

ab32127 has been referenced in 184 publications.

  • Fan XY  et al. Neonatal Sevoflurane Exposure Impairs Learning and Memory by the Hypermethylation of Hippocampal Synaptic Genes. Mol Neurobiol 58:895-904 (2021). PubMed: 33052583
  • Huang L  et al. L-3-n-Butylphthalide improves synaptic and dendritic spine plasticity and ameliorates neurite pathology in Alzheimer's disease mouse model and cultured hippocampal neurons. Mol Neurobiol 58:1260-1274 (2021). PubMed: 33146400
  • Dorigatti AO  et al. Primary neuron and astrocyte cultures from postnatal Callithrix jacchus: a non-human primate in vitro model for research in neuroscience, nervous system aging, and neurological diseases of aging. Geroscience 43:115-124 (2021). PubMed: 33063253
  • Kurioka T  et al. Decreasing auditory input induces neurogenesis impairment in the hippocampus. Sci Rep 11:423 (2021). PubMed: 33432038
  • Choi GE  et al. BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects. Nat Commun 12:487 (2021). PubMed: 33473105
View all Publications for this product

Customer reviews and Q&As

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1-10 of 37 Abreviews

Immunohistochemistry (Frozen sections) abreview for Anti-Synaptophysin antibody [YE269]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E12.5 mouse embryo)
Permeabilization
Yes - 0.25% Triton
Specification
E12.5 mouse embryo
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.2µg/mL · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Sep 29 2022

Immunohistochemistry free floating abreview for Anti-Synaptophysin antibody [YE269]

Below Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Mouse brain tissue section)
Specification
Mouse brain tissue section
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kuldeep Sachdeva

Verified customer

Submitted Sep 16 2022

Immunohistochemistry (Frozen sections) abreview for Anti-Synaptophysin antibody [YE269]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (E13.5 RAT embryo)
Permeabilization
Yes - 0.25% Triton
Specification
E13.5 RAT embryo
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Feb 09 2022

IHC - Wholemount abreview for Anti-Synaptophysin antibody [YE269]

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 Mouse Embryo)
Specification
E12.5 Mouse Embryo
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Alain Chedotal

Verified customer

Submitted Jan 31 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synaptophysin antibody [YE269]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6
Permeabilization
No
Specification
Pancreas
Blocking step
Serum from Vector Polymer Detection Kit as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
10% NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Vicki Shone

Verified customer

Submitted Jan 13 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synaptophysin antibody [YE269]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution (S1699) - 20min @ ~90oC
Permeabilization
Yes - Wash buffer, blocking solution and secondary diluent was 1X TBS with 0.1% Tween-20
Specification
Cerebellum
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
10% neutral buffered formalin
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 07 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Synaptophysin antibody [YE269]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution (S1699) - 20min @ ~90oC
Permeabilization
Yes - Wash buffer, blocking solution and secondary diluent was 1X TBS with 0.1% Tween-20
Specification
Cerebellum
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
10% neutral buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 19 2021

Immunohistochemistry free floating abreview for Anti-Synaptophysin antibody [YE269]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (striatum)
Specification
striatum
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alexandre Magno

Verified customer

Submitted Jul 01 2019

Western blot abreview for Anti-Synaptophysin antibody [YE269]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (primary culture hippocampus cell)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (15% SDS-PAGE)
Loading amount
15 µg
Treatment
RIPA & 1%Protease inhibitor for 15min on ice
Specification
primary culture hippocampus cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 06 2018

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Synaptophysin antibody [YE269]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Cynomolgus monkey Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Brain
Blocking step
Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 31 2018

1-10 of 37 Abreviews

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