Recombinant Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269] (ab206870)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [YE269] to Synaptophysin
- Suitable for: ICC/IF
- Reacts with: Rat
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 555 Anti-Synaptophysin antibody [YE269]
See all Synaptophysin primary antibodies -
Description
Alexa Fluor® 555 Rabbit monoclonal [YE269] to Synaptophysin -
Host species
Rabbit -
Conjugation
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Donkey, Cow, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab189853) -
Positive control
- ICC/IF: PC12 cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
YE269 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
- HRP Anti-Synaptophysin antibody [YE269] (ab195520)
- Alexa Fluor® 647 Anti-Synaptophysin antibody [YE269] (ab196166)
- Alexa Fluor® 488 Anti-Synaptophysin antibody [YE269] (ab196379)
- Alexa Fluor® 594 Anti-Synaptophysin antibody [YE269] (ab206868)
- Anti-Synaptophysin antibody [YE269] (ab32127)
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100.
This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min) |
Notes |
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ICC/IF
1/100. This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min) |
Target
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Function
Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. -
Tissue specificity
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. -
Involvement in disease
Mental retardation, X-linked, SYP-related -
Sequence similarities
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain. -
Domain
The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. -
Cellular localization
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 280937 Cow
- Entrez Gene: 6855 Human
- Entrez Gene: 20977 Mouse
- Entrez Gene: 24804 Rat
- Omim: 313475 Human
- SwissProt: P20488 Cow
- SwissProt: P08247 Human
- SwissProt: Q62277 Mouse
see all -
Alternative names
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
see all
Images
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ab206870 staining Synaptophysin in PC12 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206870 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab206870 has been referenced in 4 publications.
- Sulsenti R et al. Repurposing of the Antiepileptic Drug Levetiracetam to Restrain Neuroendocrine Prostate Cancer and Inhibit Mast Cell Support to Adenocarcinoma. Front Immunol 12:622001 (2021). PubMed: 33737929
- Ibarburu S et al. A Nitroalkene Benzoic Acid Derivative Targets Reactive Microglia and Prolongs Survival in an Inherited Model of ALS via NF-κB Inhibition. Neurotherapeutics 18:309-325 (2021). PubMed: 33118131
- O'Grady BJ et al. Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks. ACS Biomater Sci Eng 6:5811-5822 (2020). PubMed: 33320550
- Trias E et al. Evidence for mast cells contributing to neuromuscular pathology in an inherited model of ALS. JCI Insight 2:N/A (2017). PubMed: 29046475