Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [YE269] to Synaptophysin (HRP)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Product nameAnti-Synaptophysin antibody [YE269] (HRP)
See all Synaptophysin primary antibodies
DescriptionRabbit monoclonal [YE269] to Synaptophysin (HRP)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
- WB: PC12 cell lysate, mouse brain and rat brain lysates. IHC-P: normal human pancreas tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Synaptophysin antibody [YE269] - BSA and Azide free (ab187259)
- Anti-Synaptophysin antibody [YE269] (Alexa Fluor® 647) (ab196166)
- Anti-Synaptophysin antibody [YE269] (Alexa Fluor® 488) (ab196379)
- Anti-Synaptophysin antibody [YE269] (Alexa Fluor® 594) (ab206868)
- Anti-Synaptophysin antibody [YE269] (Alexa Fluor® 555) (ab206870)
- Anti-Synaptophysin antibody [YE269] (ab32127)
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab195520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).Can be blocked with Synaptophysin peptide (ab189853).|
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPossibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
Tissue specificityCharacteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
Involvement in diseaseMental retardation, X-linked, SYP-related
Sequence similaritiesBelongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain.
DomainThe calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
modificationsUbiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Cellular localizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, synaptosome.
- Information by UniProt
- Major synaptic vesicle protein p38 antibody
- MRX96 antibody
- MRXSYP antibody
All lanes : Anti-Synaptophysin antibody [YE269] (HRP) (ab195520) at 1/1000 dilution
Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 2 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab195520 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
IHC image of Synaptophysin staining in a section of formalin-fixed paraffin-embedded normal human pancreas tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab195520 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
ab195520 has not yet been referenced specifically in any publications.