Anti-Synaptopodin antibody (ab224491)
Key features and details
- Rabbit polyclonal to Synaptopodin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Synaptopodin antibody
See all Synaptopodin primary antibodies -
Description
Rabbit polyclonal to Synaptopodin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Synaptopodin aa 450-550.
Database link: Q8N3V7 -
Positive control
- WB: Human skeletal muscle tissue lysate. IHC-P: Human kidney, cerebral cortex and skeletal muscle tissues. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 99 kDa.
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IHC-P | (1) |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 99 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity. -
Tissue specificity
Expressed in cerebral cortex. -
Sequence similarities
Belongs to the synaptopodin family. -
Post-translational
modificationsO-glycosylated.
Isoform 2 is phosphorylated on Ser-826, Thr-836, Ser-840 and Ser-871. -
Cellular localization
Cytoplasm > cytoskeleton. Cell junction > tight junction. Perikaryon. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses. - Information by UniProt
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Database links
- Entrez Gene: 11346 Human
- Omim: 608155 Human
- SwissProt: Q8N3V7 Human
- Unigene: 435228 Human
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Alternative names
- KIAA1029 antibody
- Synaptopodin antibody
- SYNPO antibody
- SYNPO_HUMAN antibody
Images
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Paraffin-embedded human kidney tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
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Paraffin-embedded human cerebral cortex tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
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Paraffin-embedded human skeletal muscle tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
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Negative control: Paraffin-embedded human tonsil tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
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Anti-Synaptopodin antibody (ab224491) at 1/100 dilution + Human skeletal muscle tissue lysate
Developed using the ECL technique.
Predicted band size: 99 kDa -
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Synaptopodin (green) using ab224491 at 4 µg/ml in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab224491 has been referenced in 11 publications.
- Murphy C et al. Comparison of human recombinant protein coatings and fibroblast-ECM to Matrigel for induced pluripotent stem cell culture and renal podocyte differentiation. ALTEX 40:141-159 (2023). PubMed: 35791294
- Bejoy J et al. Podocytes derived from human induced pluripotent stem cells: characterization, comparison, and modeling of diabetic kidney disease. Stem Cell Res Ther 13:355 (2022). PubMed: 35883199
- Shen Q et al. Integrated genomic, transcriptomic and metabolomic analysis reveals MDH2 mutation-induced metabolic disorder in recurrent focal segmental glomerulosclerosis. Front Immunol 13:962986 (2022). PubMed: 36159820
- Wang Y et al. miR-188-3p abolishes germacrone-mediated podocyte protection in a mouse model of diabetic nephropathy in type I diabetes through triggering mitochondrial injury. Bioengineered 13:774-788 (2022). PubMed: 34847832
- Jin D et al. Impact of klotho on the expression of SRGAP2a in podocytes in diabetic nephropathy. BMC Nephrol 23:151 (2022). PubMed: 35436879