Key features and details
- Rabbit polyclonal to Synaptopodin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Synaptopodin antibody
See all Synaptopodin primary antibodies
DescriptionRabbit polyclonal to Synaptopodin
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- WB: Human skeletal muscle tissue lysate. IHC-P: Human kidney, cerebral cortex and skeletal muscle tissues. ICC/IF: U-2 OS cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 99 kDa.|
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
Tissue specificityExpressed in cerebral cortex.
Sequence similaritiesBelongs to the synaptopodin family.
Isoform 2 is phosphorylated on Ser-826, Thr-836, Ser-840 and Ser-871.
Cellular localizationCytoplasm > cytoskeleton. Cell junction > tight junction. Perikaryon. Cell projection > dendritic spine. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Localized at the tight junction of cells. In brain, localized to the postsynaptic densities and in the perikarya. Asssociated with dendritic spines of a subset of synapses.
- Information by UniProt
- KIAA1029 antibody
- Synaptopodin antibody
- SYNPO antibody
- SYNPO_HUMAN antibody
Paraffin-embedded human kidney tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral cortex tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
Negative control: Paraffin-embedded human tonsil tissue stained for Synaptopodin using ab224491 at 1/2500 dilution in immunohistochemical analysis.
Anti-Synaptopodin antibody (ab224491) at 1/100 dilution + Human skeletal muscle tissue lysate
Developed using the ECL technique.
Predicted band size: 99 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Synaptopodin (green) using ab224491 at 4 µg/ml in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224491 has been referenced in 4 publications.
- Liu DW et al. Silencing of long noncoding RNA PVT1 inhibits podocyte damage and apoptosis in diabetic nephropathy by upregulating FOXA1. Exp Mol Med 51:88 (2019). PubMed: 31371698
- Murphy C et al. A Protocol for One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes. Methods Mol Biol 1994:93-99 (2019). PubMed: 31124107
- Rauch C et al. Differentiation of human iPSCs into functional podocytes. PLoS One 13:e0203869 (2018). PubMed: 30222766
- Chen X et al. Low-dose cadmium activates the JNK signaling pathway in human renal podocytes. Int J Mol Med 41:2359-2365 (2018). PubMed: 29393374