Anti-Synaptotagmin 1 + 2 antibody (ab131548)
Key features and details
- Rabbit polyclonal to Synaptotagmin 1 + 2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Synaptotagmin 1 + 2 antibody -
Description
Rabbit polyclonal to Synaptotagmin 1 + 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region around amino acids 200-204/197-201 (
RKTLN
) of Human Synaptotagmin 1 + 2 (NP_001129277.1; NP_001129976.1). -
Positive control
- HeLa cells; HeLa cell extract
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
|
|
| ICC/IF |
1/100 - 1/200.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa. |
|
ICC/IF
1/100 - 1/200. |
Target
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Relevance
Synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. -
Cellular localization
Cytoplasmic vesicle; secretory vesicle; synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle; secretory vesicle; chromaffin granule membrane; Single-pass membrane protein. Cytoplasm -
Database links
- Entrez Gene: 127833 Human
- Entrez Gene: 6857 Human
- Entrez Gene: 20979 Mouse
- Entrez Gene: 20980 Mouse
- Entrez Gene: 24805 Rat
- Entrez Gene: 25716 Rat
- Omim: 185605 Human
- Omim: 600104 Human
see all -
Alternative names
- DKFZp781D2042 antibody
- FLJ42519 antibody
- P65 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin 1 + 2 antibody (ab131548)Immunofluorescent analysis of methanol-fixed HeLa cells labelling Synaptotagmin 1 + 2 with ab131548 at 1/100 dilution.
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Western blot - Anti-Synaptotagmin 1 + 2 antibody (ab131548)All lanes : Anti-Synaptotagmin 1 + 2 antibody (ab131548) at 1/500 dilution
Lane 1 : HeLa cell extract with blocking pepide
Lane 2 : HeLa cell extract
Predicted band size: 47 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131548 has not yet been referenced specifically in any publications.