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Anti-Synaptotagmin 1 + 2 antibody (ab131548)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin 1 + 2 antibody (ab131548)
  • Western blot - Anti-Synaptotagmin 1 + 2 antibody (ab131548)

Key features and details

  • Rabbit polyclonal to Synaptotagmin 1 + 2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Synaptotagmin 1 + 2 antibody
  • Description

    Rabbit polyclonal to Synaptotagmin 1 + 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region around amino acids 200-204/197-201 (

    RKTLN

    ) of Human Synaptotagmin 1 + 2 (NP_001129277.1; NP_001129976.1).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cells; HeLa cell extract
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
ICC/IF
1/100 - 1/200.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
ICC/IF
1/100 - 1/200.

Target

  • Relevance

    Synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.
  • Cellular localization

    Cytoplasmic vesicle; secretory vesicle; synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle; secretory vesicle; chromaffin granule membrane; Single-pass membrane protein. Cytoplasm
  • Database links

    • Entrez Gene: 127833 Human
    • Entrez Gene: 6857 Human
    • Entrez Gene: 20979 Mouse
    • Entrez Gene: 20980 Mouse
    • Entrez Gene: 24805 Rat
    • Entrez Gene: 25716 Rat
    • Omim: 185605 Human
    • Omim: 600104 Human
    • SwissProt: P21579 Human
    • SwissProt: Q8N9I0 Human
    • SwissProt: P46096 Mouse
    • SwissProt: P46097 Mouse
    • SwissProt: P21707 Rat
    • SwissProt: P29101 Rat
    • Unigene: 25422 Human
    • Unigene: 310545 Human
    • Unigene: 289702 Mouse
    • Unigene: 5102 Mouse
    • Unigene: 216272 Rat
    • Unigene: 88574 Rat
    see all
  • Alternative names

    • DKFZp781D2042 antibody
    • FLJ42519 antibody
    • P65 antibody
    • SVP65 antibody
    • synaptotagmin 1 antibody
    • synaptotagmin 2 antibody
    • Synaptotagmin I antibody
    • Synaptotagmin II antibody
    • SYT antibody
    • SYT1 antibody
    • SYT2 antibody
    • SytI antibody
    • SytII antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin 1 + 2 antibody (ab131548)
    Immunocytochemistry/ Immunofluorescence - Anti-Synaptotagmin 1 + 2 antibody (ab131548)
    Immunofluorescent analysis of methanol-fixed HeLa cells labelling Synaptotagmin 1 + 2 with ab131548 at 1/100 dilution.
  • Western blot - Anti-Synaptotagmin 1 + 2 antibody (ab131548)
    Western blot - Anti-Synaptotagmin 1 + 2 antibody (ab131548)
    All lanes : Anti-Synaptotagmin 1 + 2 antibody (ab131548) at 1/500 dilution

    Lane 1 : HeLa cell extract with blocking pepide
    Lane 2 : HeLa cell extract

    Predicted band size: 47 kDa

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab131548? Please let us know so that we can cite the reference in this datasheet.

ab131548 has not yet been referenced specifically in any publications.

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