Key features and details
- Rabbit polyclonal to Synaptotagmin
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Synaptotagmin antibody
See all Synaptotagmin primary antibodies
DescriptionRabbit polyclonal to Synaptotagmin
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from internal sequence of Human Synaptotagmin.
- Human brain cortex neurons; Extract from 293 cells treated with Forskolin
Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab133694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 47, 63 kDa.|
|IHC-P||Use a concentration of 15 µg/ml.|
FunctionMay have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
Sequence similaritiesBelongs to the synaptotagmin family.
Contains 2 C2 domains.
DomainThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2.
Cellular localizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane. Synaptic vesicles and chromaffin granules.
- Information by UniProt
- HsT1192 antibody
- MYSPC antibody
- P65 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex neurons labelling Synaptotagmin with ab133694 at 15 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
All lanes : Anti-Synaptotagmin antibody (ab133694) at 1/500 dilution
Lane 1 : Extract from 293 cells treated with Forskolin (40 nM 30')
Lane 2 : Extract from 293 cells treated with Forskolin (40 nM 30') with immunizing peptide
Predicted band size: 47, 63 kDa
ab133694 has not yet been referenced specifically in any publications.