Key features and details
- Rabbit polyclonal to Synaptotagmin
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Synaptotagmin antibody
See all Synaptotagmin primary antibodies
DescriptionRabbit polyclonal to Synaptotagmin
Specificityab51164 detects endogenous levels of total Synaptotagmin.
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthesized non-phosphopeptide derived from human Synaptotagmin around the phosphorylation site of serine 309.
- 293 cell extract treated with PMA (125ng/ml, 30min) and human breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab51164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionMay have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone.
Sequence similaritiesBelongs to the synaptotagmin family.
Contains 2 C2 domains.
DomainThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2.
Cellular localizationCytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane. Synaptic vesicles and chromaffin granules.
- Information by UniProt
- HsT1192 antibody
- MYSPC antibody
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Immunohistochemical analysis of human breast carcinoma tissue (paraffin section) using ab51164 (1/50 dilution); with and without peptide.
All lanes : Anti-Synaptotagmin antibody (ab51164) at 1/500 dilution
Lane 1 : 293 cell extract treated with
PMA (125ng/ml, 30min); without peptide
Lane 2 : 293 cell extract treated with
PMA (125ng/ml, 30min); with peptide
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
ab51164 has not yet been referenced specifically in any publications.