Anti-Synaptotagmin antibody [ASV30] (ab13259)
Key features and details
- Mouse monoclonal [ASV30] to Synaptotagmin
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat
- Isotype: IgG2a
Overview
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Product name
Anti-Synaptotagmin antibody [ASV30]
See all Synaptotagmin primary antibodies -
Description
Mouse monoclonal [ASV30] to Synaptotagmin -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Full length protein corresponding to Rat Synaptotagmin. Rat brain synaptic junction protein complexes
Database link: P21707 -
Positive control
- Mouse or Rat brain tissue extract.
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General notes
This product was changed from ascites to tissue culture supernatant on 5th July 2019. Lot numbers higher than GR3258922 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
ASV30 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000.
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
|
IP |
Use a concentration of 5 µg/ml.
|
Notes |
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WB
1/1000. |
ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use a concentration of 5 µg/ml. |
Target
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Function
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. -
Sequence similarities
Belongs to the synaptotagmin family.
Contains 2 C2 domains. -
Domain
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with Stonin 2. -
Cellular localization
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane. Synaptic vesicles and chromaffin granules. - Information by UniProt
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Database links
- Entrez Gene: 20979 Mouse
- Entrez Gene: 20980 Mouse
- Entrez Gene: 24805 Rat
- Entrez Gene: 25716 Rat
- SwissProt: P46096 Mouse
- SwissProt: P46097 Mouse
- SwissProt: P21707 Rat
- SwissProt: P29101 Rat
see all -
Alternative names
- HsT1192 antibody
- MYSPC antibody
- P65 antibody
see all
Images
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All lanes : Anti-Synaptotagmin antibody [ASV30] (ab13259) at 1/1000 dilution
Lane 1 : Molecular weight ladder
Lane 2 : Lysates prepared from mouse brain
Lane 3 : Lysates prepared from rat brainThis image was generated using the ascites version of the product.
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ICC/IF image of ab13259 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13259, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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Synaptotagmin was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Mouse monoclonal to Synaptotagmin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab13259.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 64kDa; SynaptotagminThis image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab13259 has been referenced in 22 publications.
- Lu CW et al. Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure. Biomedicines 9:N/A (2021). PubMed: 33802221
- Ivanova D et al. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv 7:N/A (2021). PubMed: 33931449
- Yi LT et al. Berberine exerts antidepressant-like effects via regulating miR-34a-synaptotagmin1/Bcl-2 axis. Chin Herb Med 13:116-123 (2021). PubMed: 36117760
- Saini J et al. A Novel Bioengineered Functional Motor Unit Platform to Study Neuromuscular Interaction. J Clin Med 9:N/A (2020). PubMed: 33050427
- Ye J et al. Tau inhibits PKA by nuclear proteasome-dependent PKAR2a elevation with suppressed CREB/GluA1 phosphorylation. Aging Cell 19:e13055 (2020). PubMed: 31668016