Key features and details
- Rabbit polyclonal to Synaptotagmin VII
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Synaptotagmin VII antibody
See all Synaptotagmin VII primary antibodies
DescriptionRabbit polyclonal to Synaptotagmin VII
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
antigen, corresponding to amino acids 51-181 of Human Synaptotagmin VII.
- Synaptotagmin VII-transfected HEK293T lysate; Human kidney tissue.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionMay be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.
Tissue specificityExpressed in a variety of adult and fetal tissues.
Sequence similaritiesBelongs to the synaptotagmin family.
Contains 2 C2 domains.
DomainThe second C2 domain/C2B is responsible for SYNCRIP binding.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane.
- Information by UniProt
- IPCA 7 antibody
- IPCA-7 antibody
- IPCA7 antibody
All lanes : Anti-Synaptotagmin VII antibody (ab121383) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : Synaptotagmin VII-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
10-20% SDS PAGE
ab121383 at 1/25 dilution staining Synaptotagmin VII in Paraffin-embedded Human kidney tissue by Immunohistochemistry, showing strong cytoplasmic positivity, with a granular pattern, in cells in tubules.
ab121383 has been referenced in 1 publication.
- Liu X et al. Synaptotagmin 7 in twist-related protein 1-mediated epithelial - Mesenchymal transition of non-small cell lung cancer. EBioMedicine 46:42-53 (2019). PubMed: 31395502