Key features and details
- Mouse monoclonal [N275/14] to Synaptotagmin VII
- Suitable for: IHC-P, IHC-Fr, ICC/IF, WB
- Reacts with: Rat
- Isotype: IgG2b
Product nameAnti-Synaptotagmin VII antibody [N275/14]
See all Synaptotagmin VII primary antibodies
DescriptionMouse monoclonal [N275/14] to Synaptotagmin VII
Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, WBmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
EpitopeCytoplasmic C2A domain
- WB: Rat brain lysate.
Abcam is committed to ensuring that our customers have access to all of the information we have on our products and that the product information is accurate. The clone name for this product was previously known as S27514. However, it has come to our attention that the original and primary clone name is N275/14 so we have therefore changed the product name accordingly.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab174633 in the following tested applications.
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/1000. Predicted molecular weight: 45 kDa.|
FunctionMay be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis.
Tissue specificityExpressed in a variety of adult and fetal tissues.
Sequence similaritiesBelongs to the synaptotagmin family.
Contains 2 C2 domains.
DomainThe second C2 domain/C2B is responsible for SYNCRIP binding.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane.
- Information by UniProt
- IPCA 7 antibody
- IPCA-7 antibody
- IPCA7 antibody
Immunocytochemistry/Immunofluorescence analysis using ab174633 at 1:100 in Neuroblastoma cell line (SK-N-BE) Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody incubaitionfor 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain at 1:1000 for 60 min RT; DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab174633 (D) Composite.
Lane 1 : Anti-Synaptotagmin VII antibody [N275/14] (ab174633) at 1/100 dilution
Lane 2 : Anti-Synaptotagmin VII antibody [N275/14] (ab174633) at 1/250 dilution
Lane 3 : Anti-Synaptotagmin VII antibody [N275/14] (ab174633) at 1/500 dilution
Lane 4 : Anti-Synaptotagmin VII antibody [N275/14] (ab174633) at 1/1000 dilution
All lanes : Rat brain lysates
Predicted band size: 45 kDa
Additional bands at: 65 kDa (possible isoform)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab174633 has not yet been referenced specifically in any publications.