Anti-SYNC antibody [OTI5B8] (ab236418)
Key features and details
- Mouse monoclonal [OTI5B8] to SYNC
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-SYNC antibody [OTI5B8] -
Description
Mouse monoclonal [OTI5B8] to SYNC -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human SYNC aa 1 to the C-terminus. Produced in E.coli (NP_110413).
Database link: Q9H7C4 -
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY SYNC cDNA. IHC-P: Human colon adenocarcinoma, kidney, liver, liver carcinoma, pancreas, thyroid carcinoma, endometrium, prostate and prostate carcinoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI5B8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 55 kDa.
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IHC-P |
1/150.
Perform heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH 8.5) at 120°C for 3 minutes. |
Notes |
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WB
1/2000. Predicted molecular weight: 55 kDa. |
IHC-P
1/150. Perform heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH 8.5) at 120°C for 3 minutes. |
Target
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Sequence similarities
Belongs to the intermediate filament family. -
Cellular localization
Cytoplasm > perinuclear region. In skeletal muscle, colocalizes with DES and DTNA, and is localized at the myotendinous and neuromuscular junctions, sarcolemma and Z-lines. In myotubes, detected in a punctate cytoplasmic pattern. - Information by UniProt
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Database links
- Entrez Gene: 81493 Human
- Omim: 611750 Human
- SwissProt: Q9H7C4 Human
- Unigene: 712631 Human
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Alternative names
- 1110057H03Rik antibody
- Intermediate filament protein syncoilin antibody
- MGC124230 antibody
see all
Images
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All lanes : Anti-SYNC antibody [OTI5B8] (ab236418) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T transfected with pCMV6-ENTRY SYNC cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 55 kDa -
Paraffin-embedded human colon adenocarcinoma tissue stained for SYNC using ab236418 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for SYNC using ab236418 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human liver tissue stained for SYNC using ab236418 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human liver carcinoma tissue stained for SYNC using ab236418 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas tissue stained for SYNC using ab236418 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human thyroid carcinoma tissue stained for SYNC using ab236418 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium tissue stained for SYNC using ab236418 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate tissue stained for SYNC using ab236418 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate carcinoma tissue stained for SYNC using ab236418 at 1/150 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236418 has not yet been referenced specifically in any publications.