Overview

  • Product name
  • Description
    Rabbit polyclonal to SynCAM
  • Host species
    Rabbit
  • Specificity
    Reacts with the C-terminal region of rat synCAM protein.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide corresponding to the C-terminal region of the rat protein.

  • Positive control
    • rat brain lysate
  • General notes


    SynCAM is a brain-specific, immunoglobulin domain. It functions as a homophilic cell adhesion molecule at the synapse. Full-length synCAM has been shown to induce synaptic assembly. It has also been shown that synCAM, in conjunction with glutamate receptors, can sufficiently create a functional postsynaptic response.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: Whole serum
  • Purity
    Whole antiserum
  • Primary antibody notes
    SynCAM is a brain-specific, immunoglobulin domain. It functions as a homophilic cell adhesion molecule at the synapse. Full-length synCAM has been shown to induce synaptic assembly. It has also been shown that synCAM, in conjunction with glutamate receptors, can sufficiently create a functional postsynaptic response.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 17548061
IHC-P Use at an assay dependent concentration. PubMed: 19643986

Target

  • Function
    Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.
  • Sequence similarities
    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Domain
    The cytoplasmic domain appears to play a critical role in proapoptosis and tumor suppressor activity in NSCLC.
  • Cellular localization
    Cell membrane. Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder.
  • Information by UniProt
  • Database links
  • Alternative names
    • BL2 antibody
    • Cadm1 antibody
    • CADM1_HUMAN antibody
    • Cell adhesion molecule 1 antibody
    • DKFZp686F1789 antibody
    • IgSF4 antibody
    • IGSF4A antibody
    • Immunoglobulin superfamily member 4 antibody
    • MGC149785 antibody
    • MGC51880 antibody
    • NECL-2 antibody
    • NECL2 antibody
    • Nectin like 2 antibody
    • Nectin-like protein 2 antibody
    • RA175 antibody
    • SgIGSF antibody
    • Spermatogenic immunoglobulin superfamily antibody
    • ST17 antibody
    • sTSLC 1 antibody
    • Synaptic cell adhesion molecule antibody
    • SynCAM antibody
    • SynCAM1 antibody
    • TSLC-1 antibody
    • TSLC1 antibody
    • Tumor suppressor in lung cancer 1 antibody
    see all

Images

  • All lanes : Anti-SynCAM antibody (ab3910) at 1/1000 dilution

    Lane 1 : rat brain lysate at 20 µg
    Lane 2 : rat brain crude synaptosomes at 20 µg
    Lane 3 : SynCAM transfected HEK293T cell lysate
    Lane 4 : HEK293T cell lysate

References

This product has been referenced in:
  • Choobineh H  et al. Testosterone Reduces Spinal Cord Injury-Induced Effects on Male Reproduction by Preventing CADM1 Defect. Cell J 20:138-149 (2018). Read more (PubMed: 29633590) »
  • Li D  et al. Armadillo repeat containing 12 promotes neuroblastoma progression through interaction with retinoblastoma binding protein 4. Nat Commun 9:2829 (2018). Read more (PubMed: 30026490) »
See all 14 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Neuroblastoma cells)
Loading amount
10 µg
Specification
Neuroblastoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 16 2009

Answer

Dear Dr. Luo, Thank you for your patience. I have received a reply from the original of ab3910. The peptide sequence is proprietary information and cannot be released. As I've already mentioned in the last e-mail, the immunogen is "a synthetic peptide corresponding to the C-terminal region of the rat protein". This antibody has not been tested for cross-reactivity with other family members or isoforms. I'm sorry that I can't provide additional information. Please don't hesitate to contact us again if you have further questions.

Read More

Answer

Thank you for your enquiry. HEK is HEK293T cells transfected with rat SynCAM. A scientist at UT Southwestern tested this antibody and said: "...the glycosylation pattern of SynCAM is different in brain and heterogously expressing cells. As you can see from the blot and in the original paper [Science. 297: 1525-1531 (2002)], Syncam in brain comes in a core glycosylated population of 50kDa and a population of higher glycosylated bands above 65kDa. When transfected in heterologous cells, only a single, glycosylated higher MW population is observed." We will update the datasheet and add the image as soon as we can,

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Answer

Thank you for your enquiry, the heterologous cell lysates tested were HEK cell lysates. We are currently sourcing the image for the blot of synCAM in the rat brain lysate and HEK cell lysate and as soon as we receive it we will add it to the datasheet, Please let us know if you require further information,

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Answer

Dear Susan, I apologize for the shortage you received. I have gone ahead and set up a free of charge replacement order to be sent you soon. You will receive an e-mail confirmation once this item is ready to be shipped to you. Kind regards, Marlen

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Answer

Thanks for your inquiry. The antibody was made to the C-terminus of the rat SynCam protein.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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