Key features and details
- Rabbit polyclonal to SynCAM/CADM1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-SynCAM/CADM1 antibody
See all SynCAM/CADM1 primary antibodies
DescriptionRabbit polyclonal to SynCAM/CADM1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Rat brain lysate; human cervical carcinoma and colon carcinoma tissues.
This product was previously labelled as SynCAM
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab216585 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 49 kDa).|
|IHC-P||1/100 - 1/500.|
FunctionMediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.
Sequence similaritiesBelongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainThe cytoplasmic domain appears to play a critical role in proapoptosis and tumor suppressor activity in NSCLC.
Cellular localizationCell membrane. Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder.
- Information by UniProt
- BL2 antibody
- Cadm1 antibody
- CADM1_HUMAN antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cervical carcinoma tissue labeling TSLC1 with ab216585 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling TSLC1 with ab216585 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
All lanes : Anti-SynCAM/CADM1 antibody (ab216585) at 1/200 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 1/3000 dilution
Predicted band size: 49 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
ab216585 has not yet been referenced specifically in any publications.