Overview

  • Product name

    Anti-SynCAM/CADM1 antibody
    See all SynCAM/CADM1 primary antibodies
  • Description

    Rabbit polyclonal to SynCAM/CADM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human SynCAM/CADM1 aa 396-442 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    GRYFARHKGTYFTHEAKGADDAADADTAIINAEGGQNNSEEKKEYFI


    Database link: Q9BY67

  • Positive control

    • Rat brain lysate; human cervical carcinoma and colon carcinoma tissues.
  • General notes

     This product was previously labelled as SynCAM

     

Properties

Applications

Our Abpromise guarantee covers the use of ab216585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 49 kDa).
IHC-P 1/100 - 1/500.

Target

  • Function

    Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and PVRL3 in a Ca(2+)-independent manner. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. May contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. May act as a synaptic cell adhesion molecule that drives synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Domain

    The cytoplasmic domain appears to play a critical role in proapoptosis and tumor suppressor activity in NSCLC.
  • Cellular localization

    Cell membrane. Associates with perinuclear and plasma membranes in vivo. Localized to the basolateral plasma membrane of epithelial cells in gall bladder.
  • Information by UniProt
  • Database links

  • Alternative names

    • BL2 antibody
    • Cadm1 antibody
    • CADM1_HUMAN antibody
    • Cell adhesion molecule 1 antibody
    • DKFZp686F1789 antibody
    • IgSF4 antibody
    • IGSF4A antibody
    • Immunoglobulin superfamily member 4 antibody
    • MGC149785 antibody
    • MGC51880 antibody
    • NECL-2 antibody
    • NECL2 antibody
    • Nectin like 2 antibody
    • Nectin-like protein 2 antibody
    • RA175 antibody
    • SgIGSF antibody
    • Spermatogenic immunoglobulin superfamily antibody
    • ST17 antibody
    • sTSLC 1 antibody
    • Synaptic cell adhesion molecule antibody
    • SynCAM antibody
    • SynCAM1 antibody
    • TSLC-1 antibody
    • TSLC1 antibody
    • Tumor suppressor in lung cancer 1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cervical carcinoma tissue labeling TSLC1 with ab216585 at 1/200 dilution, followed by secondary antibody detection and DAB staining.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling TSLC1 with ab216585 at 1/200 dilution, followed by secondary antibody detection and DAB staining.

  • All lanes : Anti-SynCAM/CADM1 antibody (ab216585) at 1/200 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L), HRP conjugated at 1/3000 dilution

    Predicted band size: 49 kDa
    Observed band size: 44 kDa
    why is the actual band size different from the predicted?

References

ab216585 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Bronchial epithelium in the lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citrate buffer pH6.0
Permeabilization
No
Specification
Bronchial epithelium in the lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 31 2018

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