Recombinant Anti-Syndecan-1 antibody [SP152] (ab130405)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)](https://www.abcam.com/ps/products/130/ab130405/Images/ab130405-5-AntiSyndecan1-antibody-SP152-IHC-Human-hepatocellular-carcinoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP152] to Syndecan-1
- Suitable for: ICC/IF, Flow Cyt, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Syndecan-1 antibody [SP152]
See all Syndecan-1 primary antibodies -
Description
Rabbit monoclonal [SP152] to Syndecan-1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Syndecan-1 aa 1-250. The exact sequence is proprietary.
Database link: P18827 -
Positive control
- IHC-P: Human tonsil, Human skin, and Human hepatocellular carcinoma tissue; ICC: A431 cells. Flow Cyt: U266B1 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP152 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
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| Flow Cyt |
1/1000.
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| IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
1/1000. |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. -
Sequence similarities
Belongs to the syndecan proteoglycan family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6382 Human
- Omim: 186355 Human
- SwissProt: P18827 Human
- Unigene: 224607 Human
- Unigene: 665958 Human
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Alternative names
- CD_antigen antibody
- CD138 antibody
- CD138 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunocytochemistry/ Immunofluorescence - Anti-Syndecan-1 antibody [SP152] (ab130405)](https://www.abcam.com/ps/products/130/ab130405/Images/ab130405-333833-anti-syndecan-1-antibody-sp152-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Syndecan-1 antibody [SP152] (ab130405)Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Syndecan-1 with purified ab130405 at 1:50 (5.36 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry - Anti-Syndecan-1 antibody [SP152] (ab130405)](https://www.abcam.com/ps/products/130/ab130405/Images/ab130405-8-anti-syndecan-1-antibody-sp152-flowcytometry-u266b1-human.jpg)
Flow Cytometry - Anti-Syndecan-1 antibody [SP152] (ab130405)Flow Cytometry analysis of U266B1 (Human multiple myeloma B lymphocyte) cells labeling Syndecan-1 with purified ab130405 at 1/1000 dilution (1 µg/mL) (Red). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Gated on viable cells. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)](https://www.abcam.com/ps/products/130/ab130405/Images/ab130405-4-AntiSyndecan1-antibody-SP152-IHC-Human-skin.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)](https://www.abcam.com/ps/products/130/ab130405/Images/ab130405-3-AntiSyndecan1-antibody-SP152-IHC-Human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)](https://www.abcam.com/ps/products/130/ab130405/Images/ab130405-144746-anti-syndecan-1-antibody-sp152-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)ab130405, at a dilution of 1/100, staining Syndecan in paraffin-embedded Human tonsil tissue by Immunohistochemistry. -
![Anti-Syndecan-1 antibody [SP152] (ab130405)](https://www.abcam.com/ps/products/130/ab130405/Images/ab130405-6-benefits-of-recombinant-antibodies.png)
Anti-Syndecan-1 antibody [SP152] (ab130405)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab130405 has been referenced in 2 publications.
- Huang X et al. Heparanase Promotes Syndecan-1 Expression to Mediate Fibrillar Collagen and Mammographic Density in Human Breast Tissue Cultured ex vivo. Front Cell Dev Biol 8:599 (2020). PubMed: 32760722
- Luo B et al. Engineering of a-PD-1 antibody-expressing long-lived plasma cells by CRISPR/Cas9-mediated targeted gene integration. Cell Death Dis 11:973 (2020). PubMed: 33184267