Recombinant
RabMAb

Recombinant Anti-Syndecan-1 antibody [SP152] (ab130405)

Rabbit recombinant monoclonal Syndecan-1 antibody [SP152]. Validated in IHC, ICC/IF and tested in Human. Immunogen corresponding to recombinant fragment.

Overview

  • Product name

    Anti-Syndecan-1 antibody [SP152]
    See all Syndecan-1 primary antibodies
  • Description

    Rabbit monoclonal [SP152] to Syndecan-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Syndecan-1 aa 1-250. The exact sequence is proprietary.
    Database link: P18827

  • Positive control

    • IHC-P: Human tonsil, Human skin, and Human hepatocellular carcinoma tissue; ICC: A431 cells.
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab130405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
  • Sequence similarities

    Belongs to the syndecan proteoglycan family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD_antigen antibody
    • CD138 antibody
    • CD138 antigen antibody
    • heparan sulfate proteoglycan fibroblast growth factor receptor antibody
    • SDC antibody
    • Sdc1 antibody
    • SDC1_HUMAN antibody
    • SYND1 antibody
    • Syndecan 1 antibody
    • Syndecan antibody
    • syndecan proteoglycan 1 antibody
    • Syndecan-1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Syndecan-1 with purified ab130405 at 1:50 (5.36 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • ab130405, at a dilution of 1/100, staining Syndecan in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

References

ab130405 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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