Recombinant
RabMAb

Recombinant Anti-Syndecan-1 antibody [SP152] (ab130405)

Rabbit recombinant monoclonal Syndecan-1 antibody [SP152]. Validated in IHC, ICC/IF and tested in Human. Immunogen corresponding to recombinant fragment.

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Overview

  • Product name

    Anti-Syndecan-1 antibody [SP152]
    See all Syndecan-1 primary antibodies

  • Description

    Rabbit monoclonal [SP152] to Syndecan-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Syndecan-1 aa 1-250. The exact sequence is proprietary.
    Database link: P18827

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human tonsil, Human skin, and Human hepatocellular carcinoma tissue; ICC: A431 cells.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Applications

Our Abpromise guarantee covers the use of ab130405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

  • Sequence similarities

    Belongs to the syndecan proteoglycan family.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD_antigen antibody
      • CD138 antibody
      • CD138 antigen antibody
      • heparan sulfate proteoglycan fibroblast growth factor receptor antibody
      • SDC antibody
      • Sdc1 antibody
      • SDC1_HUMAN antibody
      • SYND1 antibody
      • Syndecan 1 antibody
      • Syndecan antibody
      • syndecan proteoglycan 1 antibody
      • Syndecan-1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Syndecan-1 with ab130405 at 1/100 dilution (2.68 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Anti-Syndecan-1 antibody [SP152] (ab130405)
      Immunocytochemistry/ Immunofluorescence - Anti-Syndecan-1 antibody [SP152] (ab130405)
      Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Syndecan-1 with purified ab130405 at 1:50 (5.36 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [SP152] (ab130405)
      ab130405, at a dilution of 1/100, staining Syndecan in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    References

    ab130405 has not yet been referenced specifically in any publications.

    Publishing research using ab130405? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com