Overview

  • Product name

    Anti-Syndecan 2/HSPG antibody
    See all Syndecan 2/HSPG primary antibodies
  • Description

    Rabbit polyclonal to Syndecan 2/HSPG
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Syndecan 2/HSPG aa 105-122 (internal sequence).
    Sequence:

    EETDKEKVHLSDSERKMD


    Database link: P34741

  • Positive control

    • Human liver cancer tissue; HepG2, A549, U87, SKOV and Jurkat whole cell lysate (ab7899).
  • General notes

    Protein previously labeled as Syndecan 2.

Properties

Applications

Our Abpromise guarantee covers the use of ab191062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 22 kDa.

Target

  • Function

    Cell surface proteoglycan that bears heparan sulfate.
  • Sequence similarities

    Belongs to the syndecan proteoglycan family.
  • Post-translational
    modifications

    O-glycosylated with core 1 or possibly core 8 glycans. Contains heparan sulfate.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD362 antibody
    • Fibroglycan antibody
    • Heparan sulfate proteoglycan core protein antibody
    • HSPG antibody
    • SDC2 antibody
    • SDC2_HUMAN antibody
    • SYND2 antibody
    • Syndecan-2 antibody
    see all

Images

  • All lanes : Anti-Syndecan 2/HSPG antibody (ab191062) at 0.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : U87 cell lysate
    Lane 4 : SKOV cell lysate
    Lane 5 : Jurkat cell lysate

    Predicted band size: 22 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver cancer tissue labeling Syndecan 2/HSPG with ab191062 at 1μg/ml.

References

This product has been referenced in:

  • Kero D  et al. Syndecans and Enzymes Involved in Heparan Sulfate Biosynthesis and Degradation Are Differentially Expressed During Human Odontogenesis. Front Physiol 9:732 (2018). IHC-P ; Human . Read more (PubMed: 29962964) »
  • Barták BK  et al. Colorectal adenoma and cancer detection based on altered methylation pattern of SFRP1, SFRP2, SDC2, and PRIMA1 in plasma samples. Epigenetics 12:751-763 (2017). Read more (PubMed: 28753106) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH6
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Jul 07 2016

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