Product nameAnti-Syndecan 3 antibody
See all Syndecan 3 primary antibodies
DescriptionRabbit polyclonal to Syndecan 3
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
- This antibody gave a positive signal for Western Blot in Mouse Brain Tissue lysate. This antibody gave a positive signal for Immunohistochemistry (FFPE) in Mouse Brain Tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab36653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 46 kDa).|
FunctionCell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
Tissue specificityExpressed in the nervous system, the adrenal gland, and the spleen.
Sequence similaritiesBelongs to the syndecan proteoglycan family.
modificationsO-glycosylated within the Thr/Ser-rich region which could interact with lectin domains on other molecules.
- Information by UniProt
- N-syndecan antibody
- Sdc3 antibody
- SDC3_HUMAN antibody
Anti-Syndecan 3 antibody (ab36653) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
IHC image of ab36653 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36653, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab36653 has not yet been referenced specifically in any publications.