Overview

  • Product name
  • Description
    Rabbit polyclonal to Syndecan 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide derived from within residues 250 - 350 of Human Syndecan 3.

    Read Abcam's proprietary immunogen policy (Peptide available as ab36652.)

  • Positive control
    • This antibody gave a positive signal for Western Blot in Mouse Brain Tissue lysate. This antibody gave a positive signal for Immunohistochemistry (FFPE) in Mouse Brain Tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 46 kDa).

Target

  • Function
    Cell surface proteoglycan that may bear heparan sulfate (By similarity). May have a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.
  • Tissue specificity
    Expressed in the nervous system, the adrenal gland, and the spleen.
  • Sequence similarities
    Belongs to the syndecan proteoglycan family.
  • Post-translational
    modifications
    O-glycosylated within the Thr/Ser-rich region which could interact with lectin domains on other molecules.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • N-syndecan antibody
    • Sdc3 antibody
    • SDC3_HUMAN antibody
    • SDCN antibody
    • SYND3 antibody
    • Syndecan neural type antibody
    • syndecan proteoglycan 3 antibody
    • Syndecan-3 antibody
    see all

Images

  • Anti-Syndecan 3 antibody (ab36653) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes
  • IHC image of ab36653 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36653, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab36653 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your reply.

If you don't mind I will place your comment in the 'Additional notes' section of your Abreview as I think it would be helpful for other researchers.

Thank you forparticipating in our testing discount program!

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Loading amount
20 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 03 2012

Answer

Thank you for your reply.


I do apologize as there was a misunderstanding on my part regarding this antibody. I am sorry that the product was not working for your mouse samples. As you stated, the antibody is tested and guaranteed for mouse and I am happy to offer a refund.


I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website.


Regarding an alternative antibody, below please find the discount code information for testing ab63932 in flow cytometry.

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forflow cytometry with ratand include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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Answer

Thank you for your reply.


I am sorry to hear that ab36653 did not work well in your rat samples. I am glad to hear you have submitted the Abreview. We appreciate your feedback!


Regarding an alternative product, we do not have a specific antibody that is tested and guaranteed to work in WB and FC in rat. I would recommend ab63932 which is tested and guaranteed for WB in rat. We have not tested it for FC so we can't be sure that it will work in this application.


I am happy to offer another testing discount code for you to test ab63932 in FC. Please note that if you do decide to test this, you cannot redeem your current discount code to purchase this antibody. In order to qualify for a testing discount, you must purchase the antibody to be tested.


I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

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Question
Answer


I am very pleased to hear you would like to accept our offer and testab36653 in rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab36653.

To our knowledge, ab36653 has not been tested in rat. Therefore, I can offer a discount off a future purchase if you buy ab36653 now, test it inrat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab36653 in rat. I will then send a discount code. This code must be issued before purchasing ab36653 so please wait for my reply before ordering.

2. Purchase ab36653 either by phone, fax, or online (www.abcam.com).

3. Test it in rat.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab36653 turns out to be unsuitable for rat, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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