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Rabbit polyclonal to Syndecan 4
Predicted to work with:
Rat, Cow, Dog, Human, Pig, Baboon, Macaque monkey, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Syndecan 4.
This antibody gave a positive signal in both STO and NIH3T3 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
Cell surface proteoglycan that bears heparan sulfate.
Expressed in epithelial and fibroblastic cells.
Belongs to the syndecan proteoglycan family.
Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
Information by UniProt
Western blot - Anti-Syndecan 4 antibody (ab104568)
All lanes :
Anti-Syndecan 4 antibody (ab104568) at 1 µg/ml
Lane 1 :
STO (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 :
NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
18 kDa (
why is the actual band size different from the predicted?
The band observed at 18 kDa could potentially be a cleaved form of Syndecan 4 due to the presence of a 21 amino acid signal peptide.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"