Key features and details
- Rabbit polyclonal to Syndecan 4
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Syndecan 4 antibody
DescriptionRabbit polyclonal to Syndecan 4
Specificityab74139 detects endogenous levels of total Syndecan 4 protein.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic non-phosphopeptide from human Syndecan 4 around the phosphorylation site of serine 179 (E-G-SP-Y-D).
- Human brain tissue. Extracts from HepG2 cells. HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
FunctionCell surface proteoglycan that bears heparan sulfate.
Tissue specificityExpressed in epithelial and fibroblastic cells.
Sequence similaritiesBelongs to the syndecan proteoglycan family.
modificationsShedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
Cellular localizationMembrane. Secreted. Shedding of the ectodomain produces a soluble form.
- Information by UniProt
- Amphiglycan antibody
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All lanes : Anti-Syndecan 4 antibody (ab74139) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Immunohistochemistry analysis of human brain tissue using ab74139, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Immunofluorescence analysis of HeLa cells using ab74139, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
ab74139 has been referenced in 1 publication.
- Chen LL et al. SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/ß-Catenin Pathway. Mol Cells 41:853-867 (2018). PubMed: 30165731