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Neuroscience Neurotransmission Intracellular Signaling Adapters
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Anti-SynGAP antibody (ab3344)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (3)

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Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
  • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

Key features and details

  • Rabbit polyclonal to SynGAP
  • Suitable for: ICC/IF
  • Reacts with: Rat
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SynGAP antibody
    See all SynGAP primary antibodies
  • Description

    Rabbit polyclonal to SynGAP
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Rat
    See all applications and species data
  • Immunogen

    Fusion protein corresponding to Rat SynGAP aa 947-1167.
    Database link: Q9QUH6-2

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Adapters
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Rat
All applications
Human
Application Abreviews Notes
ICC/IF
Use a concentration of 1 µg/ml.
Notes
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.
  • Involvement in disease

    Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients.
  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.
  • Post-translational
    modifications

    Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption.
  • Target information above from: UniProt accession Q96PV0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8831 Human
    • Entrez Gene: 192117 Rat
    • Omim: 603384 Human
    • SwissProt: Q96PV0 Human
    • SwissProt: Q9QUH6 Rat
    • Unigene: 586264 Human
    • Unigene: 9908 Rat
    • Alternative names

      • DKFZp761G1421 antibody
      • KIAA1938 antibody
      • MRD5 antibody
      • Neuronal RasGAP antibody
      • OTTHUMP00000064825 antibody
      • p135 SynGAP antibody
      • Ras GTPase activating protein SynGAP antibody
      • Ras GTPase-activating protein SynGAP antibody
      • RASA 1 antibody
      • RASA 5 antibody
      • RASA1 antibody
      • RASA5 antibody
      • SYGP1_HUMAN antibody
      • Synaptic Ras GAP 1 antibody
      • Synaptic Ras GTPase activating protein 1 antibody
      • Synaptic Ras GTPase activating protein 1 homolog antibody
      • Synaptic Ras GTPase activating protein 135kDa antibody
      • Synaptic Ras GTPase activating protein antibody
      • Synaptic Ras GTPase-activating protein 1 antibody
      • Synaptic Ras-GAP 1 antibody
      • SYNGAP 1 antibody
      • SYNGAP1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
      Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

      Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

    • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
      Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

      Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab3344? Please let us know so that we can cite the reference in this datasheet.

    ab3344 has been referenced in 3 publications.

    • Su P  et al. Disruption of SynGAP-dopamine D1 receptor complexes alters actin and microtubule dynamics and impairs GABAergic interneuron migration. Sci Signal 12:N/A (2019). PubMed: 31387938
    • Bayés A  et al. Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins. PLoS One 7:e46683 (2012). PubMed: 23071613
    • Yang Y  et al. SynGAP moves out of the core of the postsynaptic density upon depolarization. Neuroscience : (2011). WB, Electron Microscopy ; Rat . PubMed: 21736925

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-SynGAP antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (Various ovarian cancer cell lines)
    Specification
    Various ovarian cancer cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 11 2015

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