Anti-SynGAP antibody (ab3344)
Key features and details
- Rabbit polyclonal to SynGAP
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-SynGAP antibody
See all SynGAP primary antibodies -
Description
Rabbit polyclonal to SynGAP -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Human
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Immunogen
Recombinant fragment within Rat SynGAP aa 900-1200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9QUH6-2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
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| IHC-P |
Use a concentration of 2 µg/ml.
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| Notes |
|---|
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ICC/IF
Use a concentration of 2 µg/ml. |
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IHC-P
Use a concentration of 2 µg/ml. |
Target
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Function
Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits. -
Involvement in disease
Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients. -
Sequence similarities
Contains 1 C2 domain.
Contains 1 PH domain.
Contains 1 Ras-GAP domain. -
Post-translational
modificationsPhosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption. - Information by UniProt
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Database links
- Entrez Gene: 8831 Human
- Entrez Gene: 192117 Rat
- Omim: 603384 Human
- SwissProt: Q96PV0 Human
- SwissProt: Q9QUH6 Rat
- Unigene: 586264 Human
- Unigene: 9908 Rat
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Alternative names
- DKFZp761G1421 antibody
- KIAA1938 antibody
- MRD5 antibody
see all
Images
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Immunocytochemistry - Anti-SynGAP antibody (ab3344)
Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% Triton™ X-100 permeabilized PC-12 cells staining synGAP with ab3344 at 2 µg/ml concentration and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody Alexa Fluor® 488 conjugate at 1:2000. Nuclei were stained with SlowFade® Gold Antifade Mountant with DAPI (blue), and F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin (red). Negative control is using no primary antibody control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SynGAP antibody (ab3344)Immunohistochemical anysis of paraffin-embedded human hear tissue staining synGAP with ab3344 at 1/100 dilution counterstained with hematoxylin and detected using HRP-conjugated secondary antibody. Negative control (left) has no primary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.
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Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab3344 has been referenced in 5 publications.
- Dabrowska-Bouta B et al. Early and Delayed Impact of Nanosilver on the Glutamatergic NMDA Receptor Complex in Immature Rat Brain. Int J Mol Sci 22:N/A (2021). PubMed: 33802775
- McClatchy DB et al. Quantitative analysis of global protein stability rates in tissues. Sci Rep 10:15983 (2020). PubMed: 32994440
- Su P et al. Disruption of SynGAP-dopamine D1 receptor complexes alters actin and microtubule dynamics and impairs GABAergic interneuron migration. Sci Signal 12:N/A (2019). PubMed: 31387938
- Bayés A et al. Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins. PLoS One 7:e46683 (2012). PubMed: 23071613
- Yang Y et al. SynGAP moves out of the core of the postsynaptic density upon depolarization. Neuroscience : (2011). WB, Electron Microscopy ; Rat . PubMed: 21736925
