Anti-SynGAP antibody (ab3344)

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Overview

  • Product name

    Anti-SynGAP antibody
    See all SynGAP primary antibodies

  • Description

    Rabbit polyclonal to SynGAP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human

  • Immunogen

    Fusion protein corresponding to Rat SynGAP aa 947-1167.
    Database link: Q9QUH6-2

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Primary antibody notes

    Synaptic Ras GTPase Activating Protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 148 kDa.

Target

  • Function

    Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.

  • Involvement in disease

    Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients.

  • Sequence similarities

    Contains 1 C2 domain.
    Contains 1 PH domain.
    Contains 1 Ras-GAP domain.

  • Post-translational
    modifications

    Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption.
  • Information by UniProt

  • Database links

    • Alternative names

      • DKFZp761G1421 antibody
      • KIAA1938 antibody
      • MRD5 antibody
      • Neuronal RasGAP antibody
      • OTTHUMP00000064825 antibody
      • p135 SynGAP antibody
      • Ras GTPase activating protein SynGAP antibody
      • Ras GTPase-activating protein SynGAP antibody
      • RASA 1 antibody
      • RASA 5 antibody
      • RASA1 antibody
      • RASA5 antibody
      • SYGP1_HUMAN antibody
      • Synaptic Ras GAP 1 antibody
      • Synaptic Ras GTPase activating protein 1 antibody
      • Synaptic Ras GTPase activating protein 1 homolog antibody
      • Synaptic Ras GTPase activating protein 135kDa antibody
      • Synaptic Ras GTPase activating protein antibody
      • Synaptic Ras GTPase-activating protein 1 antibody
      • Synaptic Ras-GAP 1 antibody
      • SYNGAP 1 antibody
      • SYNGAP1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
      Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

      Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

    • Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)
      Immunocytochemistry/ Immunofluorescence - Anti-SynGAP antibody (ab3344)

      Immunolocalization of SynGAP in rat hippocampal neurons using ab3344.

    References

    This product has been referenced in:

    • Su P  et al. Disruption of SynGAP-dopamine D1 receptor complexes alters actin and microtubule dynamics and impairs GABAergic interneuron migration. Sci Signal 12:N/A (2019). Read more (PubMed: 31387938) »
    • Bayés A  et al. Comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins. PLoS One 7:e46683 (2012). Read more (PubMed: 23071613) »
    See all 3 Publications for this product

    Publishing research using ab3344? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-SynGAP antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (4-12%)
    Sample
    Human Cell lysate - whole cell (Various ovarian cancer cell lines)
    Specification
    Various ovarian cancer cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 11 2015

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