Overview

  • Product name

    Anti-SynGAP antibody [EPR2883Y]
    See all SynGAP primary antibodies
  • Description

    Rabbit monoclonal [EPR2883Y] to SynGAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SynGAP (C terminal). The exact sequence is proprietary.

  • Positive control

    • Fetal brain lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab77235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 147 kDa.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.
    • Involvement in disease

      Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients.
    • Sequence similarities

      Contains 1 C2 domain.
      Contains 1 PH domain.
      Contains 1 Ras-GAP domain.
    • Post-translational
      modifications

      Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption.
    • Information by UniProt
    • Database links

    • Alternative names

      • DKFZp761G1421 antibody
      • KIAA1938 antibody
      • MRD5 antibody
      • Neuronal RasGAP antibody
      • OTTHUMP00000064825 antibody
      • p135 SynGAP antibody
      • Ras GTPase activating protein SynGAP antibody
      • Ras GTPase-activating protein SynGAP antibody
      • RASA 1 antibody
      • RASA 5 antibody
      • RASA1 antibody
      • RASA5 antibody
      • SYGP1_HUMAN antibody
      • Synaptic Ras GAP 1 antibody
      • Synaptic Ras GTPase activating protein 1 antibody
      • Synaptic Ras GTPase activating protein 1 homolog antibody
      • Synaptic Ras GTPase activating protein 135kDa antibody
      • Synaptic Ras GTPase activating protein antibody
      • Synaptic Ras GTPase-activating protein 1 antibody
      • Synaptic Ras-GAP 1 antibody
      • SYNGAP 1 antibody
      • SYNGAP1 antibody
      see all

    Images

    • All lanes : Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution

      Lane 1 : Hippocampus (Rat) Tissue Lysate
      Lane 2 : Cortex (Rat) Tissue Lysate
      Lane 3 : Hippocampus (Mouse) Tissue Lysate
      Lane 4 : Cortex (Mouse) Tissue Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 147 kDa
      Observed band size: 130,148 kDa
      why is the actual band size different from the predicted?


      Exposure time: 8 minutes


      This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab77235 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

      The doublet observed in the Western blot image is consistent with the literature (PMID:9581761).

    • Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution + fetal brain lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 147 kDa
      Observed band size: 147 kDa
      Additional bands at: 50 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:

    • Yokoi S  et al. 3'UTR Length-Dependent Control of SynGAP Isoform a2 mRNA by FUS and ELAV-like Proteins Promotes Dendritic Spine Maturation and Cognitive Function. Cell Rep 20:3071-3084 (2017). WB, ICC, IHC . Read more (PubMed: 28954225) »
    • Yang Y  et al. Camkii-mediated phosphorylation regulates distributions of Syngap-a1 and -a2 at the postsynaptic density. PLoS One 8:e71795 (2013). Electron Microscopy, WB ; Rat . Read more (PubMed: 23967245) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Question
    Answer

    Thank you very much for your interest in ab77235.

    Unfortunately, we couldn't BLAST the immunogen sequence against zebrafish since we couldn't find the zebrafish SYNGAP1 protein sequence online. If you have it, we can BLAST it agains the immunogen if you send it to us.

    To our knowledge, ab77235 has not been tested in zebrafish. Therefore, I can offer a discount off a future purchase if you buy ab77235 now, test it in zebrafish and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab77235 in zebrafish. I will then send a discount code. This code must be issued before purchasing ab77235 so please wait for my reply before ordering.
    2. Purchase ab77235 either by phone, fax, or online (www.abcam.com).
    3. Test it in zebrafish.
    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab77235 turns out to be unsuitable for zebrafish, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Question
    Answer

    We tested rat brain and did not observe a band at the predicted MW so we do not recommend the product for IP.

    Read More

    Answer

    Yes, the clone ID's are the same. Millipore sells the same SynGAP antibody.
    I hope this is helpful. Please contact us again if you have any further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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