Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2883Y] to SynGAP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-SynGAP antibody [EPR2883Y]
See all SynGAP primary antibodies
DescriptionRabbit monoclonal [EPR2883Y] to SynGAP
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SynGAP (C terminal). The exact sequence is proprietary.
- Fetal brain lysate
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab77235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 147 kDa.|
FunctionMajor constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.
Involvement in diseaseDefects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5) [MIM:612621]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. MRD5 patients show global developmental delay with delayed motor development, hypotonia, moderate-to-severe mental retardation, and severe language impairment. Autism can be present in some patients.
Sequence similaritiesContains 1 C2 domain.
Contains 1 PH domain.
Contains 1 Ras-GAP domain.
modificationsPhosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption.
- Information by UniProt
- DKFZp761G1421 antibody
- KIAA1938 antibody
- MRD5 antibody
All lanes : Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution
Lane 1 : Hippocampus (Rat) Tissue Lysate
Lane 2 : Cortex (Rat) Tissue Lysate
Lane 3 : Hippocampus (Mouse) Tissue Lysate
Lane 4 : Cortex (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 147 kDa
Observed band size: 130,148 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab77235 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
The doublet observed in the Western blot image is consistent with the literature (PMID:9581761).
Anti-SynGAP antibody [EPR2883Y] (ab77235) at 1/1000 dilution + fetal brain lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 147 kDa
Observed band size: 147 kDa
Additional bands at: 50 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
ab77235 has been referenced in 3 publications.
- Yokoi S et al. 3'UTR Length-Dependent Control of SynGAP Isoform a2 mRNA by FUS and ELAV-like Proteins Promotes Dendritic Spine Maturation and Cognitive Function. Cell Rep 20:3071-3084 (2017). WB, ICC, IHC . PubMed: 28954225
- Yang Y et al. Camkii-mediated phosphorylation regulates distributions of Syngap-a1 and -a2 at the postsynaptic density. PLoS One 8:e71795 (2013). Electron Microscopy, WB ; Rat . PubMed: 23967245
- McMahon AC et al. SynGAP isoforms exert opposing effects on synaptic strength. Nat Commun 3:900 (2012). WB, IF ; Mouse . PubMed: 22692543