Overview

  • Product name

    Anti-Synip antibody [EP302Y]
  • Description

    Rabbit monoclonal [EP302Y] to Synip
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for Synip. It is predicted to detect the isoform 2 based an sequence analysis.
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Synip (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human skeletal lysate and human lung carcinoma tissue.
  • General notes

    Previously labelled as Syntaxin-binding protein 4. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab40674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 68 kDa (predicted molecular weight: 61 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose.
    • Sequence similarities

      Contains 1 PDZ (DHR) domain.
      Contains 1 WW domain.
    • Post-translational
      modifications

      Phosphorylated on Ser-99 by PKB/AKT2 after insulin treatment. Phosphorylation on Ser-99 abolishes the interaction with STX4A (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ16496 antibody
      • MGC149829 antibody
      • MGC50337 antibody
      • OTTHUMP00000082667 antibody
      • OTTHUMP00000227400 antibody
      • STX4-interacting protein antibody
      • STXB4_HUMAN antibody
      • Stxbp4 antibody
      • Synip antibody
      • Syntaxin 4 interacting protein antibody
      • Syntaxin 4-interacting protein antibody
      • Syntaxin binding protein 4 antibody
      • Syntaxin-binding protein 4 antibody
      see all

    Images

    • ab40674 at a 1:250 dilution staining Synip in human lung carcinoma.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Synip antibody [EP302Y] (ab40674) at 1/5000 dilution + human skeletal lysate

      Predicted band size: 61 kDa
      Observed band size: 68 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Otaka Y  et al. STXBP4 Drives Tumor Growth and Is Associated with Poor Prognosis through PDGF Receptor Signaling in Lung Squamous Cell Carcinoma. Clin Cancer Res 23:3442-3452 (2017). IHC . Read more (PubMed: 28087642) »
    • Taniai E  et al. Crosstalk between PTEN/Akt2 and TGFbeta signaling involving EGF receptor down-regulation during the tumor promotion process from the early stage in a rat two-stage hepatocarcinogenesis model. Cancer Sci 100:813-20 (2009). IHC-P ; Rat . Read more (PubMed: 19309364) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for contacting us.

    The immunogen sequence of anti TRAIL antibody used in this kit corresponds to 114-281 of human TRAIL. It is more likely that it will detect your partial N-terminus (aa114-281) protein.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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