Anti-Synphilin 1 antibody (ab217373)
Key features and details
- Rabbit polyclonal to Synphilin 1
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Synphilin 1 antibody
See all Synphilin 1 primary antibodies -
Description
Rabbit polyclonal to Synphilin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human -
Immunogen
Synthetic peptide within Human Synphilin 1 aa 61-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9Y6H5 -
Positive control
- Rat brain tissue or Rat brain whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/500.
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WB |
1/100 - 1/1000. Predicted molecular weight: 100 kDa.
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Notes |
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IHC-P
1/100 - 1/500. |
WB
1/100 - 1/1000. Predicted molecular weight: 100 kDa. |
Target
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Function
Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1. -
Tissue specificity
Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta. -
Involvement in disease
Parkinson disease -
Sequence similarities
Contains 6 ANK repeats. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation. -
Cellular localization
Cytoplasm. Detected in cytoplasmic inclusion bodies, together with SNCA. - Information by UniProt
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Database links
- Entrez Gene: 9627 Human
- Entrez Gene: 67847 Mouse
- Entrez Gene: 307309 Rat
- Omim: 603779 Human
- SwissProt: Q9Y6H5 Human
- SwissProt: Q99ME3 Mouse
- Unigene: 426463 Human
- Unigene: 292168 Mouse
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Alternative names
- Alpha synuclein interacting protein antibody
- Alpha-synuclein-interacting protein antibody
- MGC39814 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat brain tissue labeling Synphilin 1 with ab217373 at a 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.
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Anti-Synphilin 1 antibody (ab217373) at 1/200 dilution + Rat brain whole cell lysate
Secondary
Goat Anti-Goat Anti-Rabbit IgG (H+L) HRP at 1/3000 dilution
Predicted band size: 100 kDa
Additional bands at: 60kDa, 100 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab217373 has not yet been referenced specifically in any publications.