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Does this antibody detect Synphilin in Western blotting in whole brain homogenates and how many bands are there? Does it recognize mouse Synphilin?
Asked on Nov 02 2000
Synphilin-1 cannot be detected in whole brain homogenates without immunoprecipitation first. When the antibody is used to immunoprecipitate material from whole rat brain, the major band is at approximately 90kDa and faint bands at approximately 60 and 40kDa. Mouse tissue has never been tested by us.
Answered on Nov 16 2000