Product nameSynphilin 1 overexpression 293T lysate (whole cell)
DescriptionSynphilin 1 overexpression lysate
ab94102 is a 293T cell transfected lysate in which Human Synphilin 1 has been transiently over-expressed using a pCMV-Synphilin 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundDisease: Parkinson disease Function: Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1. PTM: Ubiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation. Similarity: Contains 6 ANK repeats. Tissue specificity: Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta.
Our Abpromise guarantee covers the use of ab94102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94102 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Synphilin 1 antibody (ab56627) at 1/500 dilution
Lane 1 :
Synphilin 1 overexpression 293T lysate (whole cell) (ab94102)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94102 has not yet been referenced specifically in any publications.