Anti-Syntaxin 12/Syntaxin-13 antibody (ab220746)
Key features and details
- Rabbit polyclonal to Syntaxin 12/Syntaxin-13
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Syntaxin 12/Syntaxin-13 antibody
See all Syntaxin 12/Syntaxin-13 primary antibodies -
Description
Rabbit polyclonal to Syntaxin 12/Syntaxin-13 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Orangutan -
Immunogen
Recombinant fragment corresponding to Human Syntaxin 12/Syntaxin-13 aa 100-200.
Database link: Q86Y82 -
Positive control
- RT-4 and U-251 MG cell line lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 32 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 32 kDa. |
Target
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Function
SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network membrane. - Information by UniProt
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Database links
- Entrez Gene: 23673 Human
- Entrez Gene: 100226 Mouse
- Entrez Gene: 100172323 Orangutan
- Entrez Gene: 65033 Rat
- Omim: 606892 Human
- SwissProt: Q86Y82 Human
- SwissProt: Q9ER00 Mouse
- SwissProt: Q5RBW6 Orangutan
see all -
Alternative names
- MGC51957 antibody
- Stx12 antibody
- STX12_HUMAN antibody
see all
Images
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All lanes : Anti-Syntaxin 12/Syntaxin-13 antibody (ab220746) at 1/500 dilution
Lane 1 : RT-4 cell line lysate
Lane 2 : U-251 MG cell line lysate
Predicted band size: 32 kDa -
Immunofluorescent analysis of PFA-fixed/Triton X-100 permeabilized HeLa cells labeling Syntaxin 12/Syntaxin-13 with ab220746 at 4 μg/ml (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab220746 has not yet been referenced specifically in any publications.