Product nameAnti-Syntaxin 16 antibody - Drosophila Golgi/Trans-Golgi Marker
See all Syntaxin 16 primary antibodies
DescriptionRabbit polyclonal to Syntaxin 16 - Drosophila Golgi/Trans-Golgi Marker
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Drosophila melanogaster
- Schneider L2 (Fruit fly (Drosophila melanogaster)) Whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab32340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 20935638|
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).Can be blocked with D. melanogaster Syntaxin 16 peptide (ab32339).|
FunctionSNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
Involvement in diseaseGenetic variations in STX16 may be a cause of pseudohypoparathyroidism type 1B (PHP1B) [MIM:603233]. Pseudohypoparathyroidism refers to a heterogeneous group of disorders characterized by resistance to parathyroid hormone (PTH). PHP1B is characterized by PTH-resistant hypocalcemia and hyperphosphatemia. Patients affected with PHP1B lack developmental defects characteristic of Albright hereditary osteodystrophy, and typically show no other endocrine abnormalities besides resistance to PTH. In some cases microdeletions involving STX16 appear to cause loss of methylation at exon A/B of the GNAS gene, resulting in PHP1B.
Sequence similaritiesBelongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
Cellular localizationCytoplasm and Golgi apparatus membrane.
- Information by UniProt
- hsyn16 antibody
- MGC90328 antibody
- Stx16 antibody
Anti-Syntaxin 16 antibody - Drosophila Golgi/Trans-Golgi Marker (ab32340) at 1 µg/ml +
Schneider L2 whole cell lysate (ab14893) at 20 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
This product has been referenced in:
- Charng WL et al. Drosophila Tempura, a novel protein prenyltransferase a subunit, regulates notch signaling via Rab1 and Rab11. PLoS Biol 12:e1001777 (2014). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 24492843) »
- Hornbruch-Freitag C et al. Drosophila Swiprosin-1/EFHD2 accumulates at the prefusion complex stage during Drosophila myoblast fusion. J Cell Sci 124:3266-78 (2011). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 21896648) »