Recombinant Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
![Western blot - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)](https://www.abcam.com/ps/products/134/ab134945/Images/ab134945-149147-anti-syntaxin-16-antibody-epr9156-western-blot.jpg "Western blot - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9156] to Syntaxin 16
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Syntaxin 16 antibody [EPR9156]
See all Syntaxin 16 primary antibodies -
Description
Rabbit monoclonal [EPR9156] to Syntaxin 16 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Syntaxin 16 aa 50-150. The exact sequence is proprietary.
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Positive control
- Human testis and lung adenocarcinoma tissues; HepG2 cells; 293T, HeLa, HepG2 and SH-SY5Y cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9156 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134945 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
1/100 - 1/250.
|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/250. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Genetic variations in STX16 may be a cause of pseudohypoparathyroidism type 1B (PHP1B) [MIM:603233]. Pseudohypoparathyroidism refers to a heterogeneous group of disorders characterized by resistance to parathyroid hormone (PTH). PHP1B is characterized by PTH-resistant hypocalcemia and hyperphosphatemia. Patients affected with PHP1B lack developmental defects characteristic of Albright hereditary osteodystrophy, and typically show no other endocrine abnormalities besides resistance to PTH. In some cases microdeletions involving STX16 appear to cause loss of methylation at exon A/B of the GNAS gene, resulting in PHP1B. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Cytoplasm and Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 8675 Human
- Entrez Gene: 228960 Mouse
- Entrez Gene: 362283 Rat
- Omim: 603666 Human
- SwissProt: O14662 Human
- SwissProt: Q8BVI5 Mouse
- Unigene: 307913 Human
- Unigene: 277977 Mouse
see all -
Alternative names
- hsyn16 antibody
- MGC90328 antibody
- Stx16 antibody
see all
Images
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Western blot - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)All lanes : Anti-Syntaxin 16 antibody [EPR9156] (ab134945) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)](https://www.abcam.com/ps/products/134/ab134945/Images/ab134945-149150-anti-syntaxin-16-antibody-epr9156-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)Immunofluorescence staining of HepG2 cells, labelling Syntaxin 16 using ab134945 at a 1/100 dilution (red). Cell nuclei are shown in blue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)](https://www.abcam.com/ps/products/134/ab134945/Images/ab134945-149149-anti-syntaxin-16-antibody-epr9156-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung adenocarcinoma tissue, labelling Syntaxin 16 using ab134945 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)](https://www.abcam.com/ps/products/134/ab134945/Images/ab134945-149148-anti-syntaxin-16-antibody-epr9156-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human testis tissue, labelling Syntaxin 16 using ab134945 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Syntaxin 16 antibody [EPR9156] (ab134945)](https://www.abcam.com/ps/products/134/ab134945/Images/ab134945-5-benefits-of-recombinant-antibodies.png)
Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab134945 has been referenced in 1 publication.
- Carpier JM et al. Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation. J Exp Med 215:1245-1265 (2018). PubMed: 29440364