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RecombinantRabMAb

Recombinant Anti-Syntaxin 16 antibody [EPR9156] (ab134945)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (1)

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Western blot - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
  • Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
  • Anti-Syntaxin 16 antibody [EPR9156] (ab134945)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9156] to Syntaxin 16
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Syntaxin 16 antibody [EPR9156]
    See all Syntaxin 16 primary antibodies
  • Description

    Rabbit monoclonal [EPR9156] to Syntaxin 16
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Syntaxin 16 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human testis and lung adenocarcinoma tissues; HepG2 cells; 293T, HeLa, HepG2 and SH-SY5Y cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9156
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Golgi

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Syntaxin 16 antibody [EPR9156] (ab225186)
    • Alexa Fluor® 488 Anti-Syntaxin 16 antibody [EPR9156] (ab225187)
    • Anti-Syntaxin 16 antibody [EPR9156] - BSA and Azide free (ab238922)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 cytoplasmic extract lysate (ab14659)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
ICC/IF (2)
1/100 - 1/250.
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
    • Tissue specificity

      Ubiquitous.
    • Involvement in disease

      Genetic variations in STX16 may be a cause of pseudohypoparathyroidism type 1B (PHP1B) [MIM:603233]. Pseudohypoparathyroidism refers to a heterogeneous group of disorders characterized by resistance to parathyroid hormone (PTH). PHP1B is characterized by PTH-resistant hypocalcemia and hyperphosphatemia. Patients affected with PHP1B lack developmental defects characteristic of Albright hereditary osteodystrophy, and typically show no other endocrine abnormalities besides resistance to PTH. In some cases microdeletions involving STX16 appear to cause loss of methylation at exon A/B of the GNAS gene, resulting in PHP1B.
    • Sequence similarities

      Belongs to the syntaxin family.
      Contains 1 t-SNARE coiled-coil homology domain.
    • Cellular localization

      Cytoplasm and Golgi apparatus membrane.
    • Target information above from: UniProt accession O14662 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8675 Human
      • Entrez Gene: 228960 Mouse
      • Entrez Gene: 362283 Rat
      • Omim: 603666 Human
      • SwissProt: O14662 Human
      • SwissProt: Q8BVI5 Mouse
      • Unigene: 307913 Human
      • Unigene: 277977 Mouse
      • Unigene: 55471 Rat
      see all
    • Alternative names

      • hsyn16 antibody
      • MGC90328 antibody
      • Stx16 antibody
      • STX16_HUMAN antibody
      • Syn16 antibody
      • Syntaxin-16 antibody
      see all

    Images

    • Western blot - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Western blot - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      All lanes : Anti-Syntaxin 16 antibody [EPR9156] (ab134945) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 37 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Immunofluorescence staining of HepG2 cells, labelling Syntaxin 16 using ab134945 at a 1/100 dilution (red). Cell nuclei are shown in blue.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)

      Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung adenocarcinoma tissue, labelling Syntaxin 16 using ab134945 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)

      Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human testis tissue, labelling Syntaxin 16 using ab134945 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Anti-Syntaxin 16 antibody [EPR9156] (ab134945)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab134945? Please let us know so that we can cite the reference in this datasheet.

    ab134945 has been referenced in 1 publication.

    • Carpier JM  et al. Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation. J Exp Med 215:1245-1265 (2018). PubMed: 29440364

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Syntaxin 16 antibody [EPR9156]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (INS-1 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    INS-1 cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Dr. Jerome Cattin

    Verified customer

    Submitted Nov 03 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Syntaxin 16 antibody [EPR9156]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - Triton X100 0.5%
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Jerome Cattin

    Verified customer

    Submitted Oct 29 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Syntaxin 16 antibody [EPR9156]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (INS1 cells)
    Specification
    INS1 cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Jerome Cattin

    Verified customer

    Submitted Oct 29 2020

    Western blot abreview for Anti-Syntaxin 16 antibody [EPR9156]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (hek293T)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    hek293T
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 14 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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