Recombinant
RabMAb

Recombinant Anti-Syntaxin 16 antibody [EPR9156] (ab134945)

Submit a review Submit a question References (1)

Overview

  • Product name

    Anti-Syntaxin 16 antibody [EPR9156]
    See all Syntaxin 16 primary antibodies

  • Description

    Rabbit monoclonal [EPR9156] to Syntaxin 16

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Syntaxin 16 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human testis and lung adenocarcinoma tissues; HepG2 cells; 293T, HeLa, HepG2 and SH-SY5Y cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9156

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.

    • Tissue specificity

      Ubiquitous.

    • Involvement in disease

      Genetic variations in STX16 may be a cause of pseudohypoparathyroidism type 1B (PHP1B) [MIM:603233]. Pseudohypoparathyroidism refers to a heterogeneous group of disorders characterized by resistance to parathyroid hormone (PTH). PHP1B is characterized by PTH-resistant hypocalcemia and hyperphosphatemia. Patients affected with PHP1B lack developmental defects characteristic of Albright hereditary osteodystrophy, and typically show no other endocrine abnormalities besides resistance to PTH. In some cases microdeletions involving STX16 appear to cause loss of methylation at exon A/B of the GNAS gene, resulting in PHP1B.

    • Sequence similarities

      Belongs to the syntaxin family.
      Contains 1 t-SNARE coiled-coil homology domain.

    • Cellular localization

      Cytoplasm and Golgi apparatus membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • hsyn16 antibody
      • MGC90328 antibody
      • Stx16 antibody
      • STX16_HUMAN antibody
      • Syn16 antibody
      • Syntaxin-16 antibody
      see all

    Images

    • Western blot - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Western blot - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      All lanes : Anti-Syntaxin 16 antibody [EPR9156] (ab134945) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 37 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Immunocytochemistry/ Immunofluorescence - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Immunofluorescence staining of HepG2 cells, labelling Syntaxin 16 using ab134945 at a 1/100 dilution (red). Cell nuclei are shown in blue.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)

      Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human lung adenocarcinoma tissue, labelling Syntaxin 16 using ab134945 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 16 antibody [EPR9156] (ab134945)

      Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human testis tissue, labelling Syntaxin 16 using ab134945 at a 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Carpier JM  et al. Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation. J Exp Med 215:1245-1265 (2018). Read more (PubMed: 29440364) »
    See 1 Publication for this product

    Publishing research using ab134945? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab134945.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com