Product nameAnti-Syntaxin 1a antibody
See all Syntaxin 1a primary antibodies
DescriptionRabbit polyclonal to Syntaxin 1a
SpecificityUnfortunately we can not exlude crossreactivity with the very homologue protein Syntaxin 1b as the alignement score of the immunogen is relatively high with mouse Syntaxin 1b.
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Chicken, Human, Zebrafish
- Rat or Mouse Brain Tissue Extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab13262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35-36 kDa (predicted molecular weight: 34.6 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
FunctionPotentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
Tissue specificityIsoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
Involvement in diseaseNote=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
Cellular localizationSecreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
- Information by UniProt
- HPC 1 antibody
- Neuron specific antigen HPC1 antibody
- Neuron-specific antigen HPC-1 antibody
All lanes : Anti-Syntaxin 1a antibody (ab13262) at 1/2000 dilution
Lane 1 : Chicken brain tissue lysate
Lane 2 : Chicken DRG tissue lysate
Lysates/proteins at 40 µg per lane.
All lanes : HRP-conjugated donkey anti-rabbit IgG at 1/10000 dilution
Performed under non-reducing conditions.
Predicted band size: 34.6 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 150 seconds
ab13262 has not yet been referenced specifically in any publications.