• Product name
  • Description
    Rabbit polyclonal to Syntaxin 1a
  • Host species
  • Specificity
    Unfortunately we can not exlude crossreactivity with the very homologue protein Syntaxin 1b as the alignement score of the immunogen is relatively high with mouse Syntaxin 1b.
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Chicken, Human, Zebrafish
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 1-19 of Human Syntaxin.

  • Positive control
    • Rat or Mouse Brain Tissue Extract.



Our Abpromise guarantee covers the use of ab13262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35-36 kDa (predicted molecular weight: 34.6 kDa).
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.


  • Function
    Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
  • Tissue specificity
    Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
  • Involvement in disease
    Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities
    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Cellular localization
    Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • HPC 1 antibody
    • Neuron specific antigen HPC1 antibody
    • Neuron-specific antigen HPC-1 antibody
    • OTTHUMP00000174615 antibody
    • OTTHUMP00000174616 antibody
    • OTTHUMP00000174617 antibody
    • OTTHUMP00000174618 antibody
    • P35-1 antibody
    • STX1 antibody
    • STX1A antibody
    • STX1A_HUMAN antibody
    • SYN1A antibody
    • Syntaxin 1A (brain) antibody
    • Syntaxin 1A brain antibody
    • Syntaxin-1A antibody
    see all


  • All lanes : Anti-Syntaxin 1a antibody (ab13262) at 1/2000 dilution

    Lane 1 : Chicken brain tissue lysate
    Lane 2 : Chicken DRG tissue lysate

    Lysates/proteins at 40 µg per lane.

    All lanes : HRP-conjugated donkey anti-rabbit IgG at 1/10000 dilution

    Performed under non-reducing conditions.

    Predicted band size: 34.6 kDa
    Observed band size: 34 kDa
    why is the actual band size different from the predicted?

    Exposure time: 150 seconds

    See Abreview


ab13262 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Denaturing (Extra PAGE ONE 5-20%)
Chicken Tissue lysate - whole (Brain and DRG)
Brain and DRG
Blocking step
Blocking one as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 13 2015


Thank you very much for your patience while I was in contact with the laboratory. Please find here the alignment or comments to our other Syntaxin-1a antibodies. It was in the end more difficult than expected to find an antibody which I could recommend. In summary, I would recommend the ab103179 Click here (or use the following: https://www.abcam.com/index.html?datasheet=103179).  Indeed, I have contacted the producers of ab103179 - another anti Syntaxin-1a antibody. The laboratory has run the alignment with the mouse 1b sequence and confirmed that the antibody is specific for syntaxin 1a. Indeed, only 7 out 14 amino acids of the immunogen sequence matched (50% homology). However the ab103179 has not yet been tested for reactivity on mouse tissue,  If you are interested to test this antibody yourself on the mouse tissue, I would be happy to make you a testing discount offer (www.abcam.com/collaborationdiscount) Please let me know if this is of interest to you. Unfortunately ab13262 has been tested against mouse and in WB only - immunogen MKDRTQELRTAKDSDDDDD  - alignment however relative high with Syntaxin 1b (*********:******::: )  http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111019-125408-0203-93391256-pg I have added a note on the datasheet.  The immunogen sequence of ab41353 is unfortunately also very conserved with Syntaxin 1b and we already had a note on the datasheet in this regards. As ab78539 is made against the whole protein, I can not really recommend this neither. I am sorry for not having more choice for you. Please let me know whether you would like to have further information or the discount code for ab103179.

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