Recombinant Anti-Syntaxin 1a antibody [EPR11072(B)] (ab170890)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11072(B)] to Syntaxin 1a
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Syntaxin 1a antibody [EPR11072(B)]
See all Syntaxin 1a primary antibodies -
Description
Rabbit monoclonal [EPR11072(B)] to Syntaxin 1a -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Syntaxin 1a aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q16623 -
Positive control
- Fetal brain, Human cerebellum and SH-SY5Y lysates; SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11072(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
1/10 - 1/100.
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WB |
1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/10 - 1/100. |
WB
1/10000 - 1/50000. Predicted molecular weight: 33 kDa. |
Target
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Function
Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. -
Tissue specificity
Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain. -
Involvement in disease
Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 6804 Human
- Entrez Gene: 20907 Mouse
- Entrez Gene: 116470 Rat
- Omim: 186590 Human
- SwissProt: Q16623 Human
- SwissProt: O35526 Mouse
- SwissProt: P32851 Rat
- Unigene: 647024 Human
see all -
Alternative names
- HPC 1 antibody
- Neuron specific antigen HPC1 antibody
- Neuron-specific antigen HPC-1 antibody
see all
Images
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All lanes : Anti-Syntaxin 1a antibody [EPR11072(B)] (ab170890) at 1/10000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : Human cerebellum lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa -
Western blot analysis on immunoprecipitation pellet from human fetal brain lysate using ab170890.
Lane 1 Primary: ab170890, 1/10000 dilution
Sample: Immunoprecipitation pellet from Human fetal brain lysatePredicted band 33 kDa
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Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells labeling Syntaxin 1a with ab170890 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab170890 has not yet been referenced specifically in any publications.