Product nameAnti-Syntaxin 1a antibody [EPR11072(B)]
See all Syntaxin 1a primary antibodies
DescriptionRabbit monoclonal [EPR11072(B)] to Syntaxin 1a
Tested applicationsSuitable for: IP, Flow Cyt, WBmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Syntaxin 1a aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q16623
- Fetal brain, Human cerebellum and SH-SY5Y lysates; SH-SY5Y cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/10000 - 1/50000. Predicted molecular weight: 33 kDa.|
FunctionPotentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
Tissue specificityIsoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
Involvement in diseaseNote=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
Cellular localizationSecreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
- Information by UniProt
- HPC 1 antibody
- Neuron specific antigen HPC1 antibody
- Neuron-specific antigen HPC-1 antibody
All lanes : Anti-Syntaxin 1a antibody [EPR11072(B)] (ab170890) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human cerebellum lysate
Lane 3 : SH-SY5Y lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa
Western blot analysis on immunoprecipitation pellet from human fetal brain lysate using ab170890.
Lane 1 Primary: ab170890, 1/10000 dilution
Sample: Immunoprecipitation pellet from Human fetal brain lysate
Predicted band 33 kDa
Flow cytometric analysis of permeabilized SH-SY5Y cells labeling Syntaxin 1a with ab170890 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
ab170890 has not yet been referenced specifically in any publications.