• Product name

    Anti-Syntaxin 2/STX2 antibody
    See all Syntaxin 2/STX2 primary antibodies
  • Description

    Rabbit polyclonal to Syntaxin 2/STX2
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IP, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Human, Pig, Xenopus laevis, Drosophila melanogaster, Monkey
  • Immunogen

    Synthetic peptide corresponding to Rat Syntaxin 2/STX2 aa 1-19 conjugated to keyhole limpet haemocyanin.


  • Positive control

    • HeLa Cell Lysate, Rat Brain Tissue Extract, Mouse Brain Tissue Extract.
  • General notes

     This product was previously labelled as Syntaxin 2



  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    This antibody was purified on a peptide bound immunoaffinity column.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab12369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33.3 kDa). human and 1/1000-1/2000 for other species.
IP Use a concentration of 10 µg/ml.
ICC Use at an assay dependent dilution.


  • Function

    Essential for epithelial morphogenesis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
  • Sequence similarities

    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Form

    Inhibition of syntaxin-2 and Vamp8 function by overexpression of nonmembrane-anchored mutants caused failure of cytokinesis leading to the formation of binucleated cells. There are 3 isoforms produced by alternative splicing.
  • Alternative names

    • EPIM antibody
    • Epimorphin antibody
    • EPM antibody
    • MGC51014 antibody
    • OTTHUMP00000238636 antibody
    • OTTHUMP00000238637 antibody
    • Stx2 antibody
    • STX2_HUMAN antibody
    • STX2A antibody
    • STX2B antibody
    • STX2C antibody
    • Syntaxin 2A antibody
    • Syntaxin 2B antibody
    • Syntaxin 2C antibody
    • Syntaxin-2 antibody
    see all


  • All lanes : Anti-Syntaxin 2/STX2 antibody (ab12369) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human skin tissue lysate - total protein (ab30166)

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33.3 kDa
    Observed band size: 33.3 kDa
    Additional bands at: 74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 4 minutes
  • ICC/IF image of ab12369 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12369, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Shi L  et al. HIV and HCV Co-Culture Promotes Profibrogenic Gene Expression through an Epimorphin-Mediated ERK Signaling Pathway in Hepatic Stellate Cells. PLoS One 11:e0158386 (2016). WB . Read more (PubMed: 27362846) »
  • Roqueta-Rivera M  et al. Deficiency in the omega-3 fatty acid pathway results in failure of acrosome biogenesis in mice. Biol Reprod 85:721-32 (2011). Read more (PubMed: 21653892) »
See all 5 Publications for this product

Customer reviews and Q&As


Thank you for your enquiry which has been forwarded to me as my colleague Karin, who you originally spoke to, is not available in the office at the moment. I am sorry to confirm that we do not sell larger vial sizes of ab12369 Anti-Syntaxin 2 antibody. You would need to order the relevant number of vials to make up how much you want. Although we would recommend to try one vial before ordering any more, we can offer a discount if you purchase 5 vials or more on one order. Please contact our customer service team with details of how many vials you require and they will be able to provide you with a quote (orders@abcam.com). Also, when you place your order, if you would like the vials to be from the same batch please let us know and we can let you know if this is possible at the time of ordering. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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