Overview

  • Product name
  • Description
    Rabbit polyclonal to Syntaxin 3
  • Host species
    Rabbit
  • Specificity
    Reacts with 35 kDa protein.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Xenopus laevis
    Predicted to work with: Mammals
  • Immunogen

    Fusion protein (Rat).

Properties

Applications

Our Abpromise guarantee covers the use of ab4113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000.
IHC-P Use at an assay dependent concentration. PubMed: 17255364
ICC/IF Use at an assay dependent concentration.

Target

References

This product has been referenced in:
See all 12 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (well-differentiated primary bronchial epithelial c)
Specification
well-differentiated primary bronchial epithelial c
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 0.1% in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C

Mr. silvia kreda

Verified customer

Submitted Dec 07 2007

Answer

I'm sorry to hear you are experiencing a problem with ab4113. I would recommend two changes: 1) in order to avoid non specific interaction, please centrifuge the antisera before use around 2000g for 10 minutes. 2) the blocking buffer may be also responsible if you use milk or BSA not filtered, try filtered 5% BSA for 1hr in TBST and incubating the antibodies in TBST only. The antibody has been used in the following publication, maybe this will help you too: Galli T et al. A novel tetanus neurotoxin-insensitive vesicle-associated membrane protein in SNARE complexes of the apical plasma membrane of epithelial cells. Mol Biol Cell 9:1437-48 (1998). I hope these suggestions will help you, as the antibody was purchased more than 90days ago we will not be able to offer you a replacement,

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Answer

The Galli T et al. paper is the only one that we are aware that specifically features the use of this antibody. As you have no doubt seen for correspondence with previous customers posted on the enquiries page of the online datasheet, this antibody has not been produced at Abcam or tested in our lab. However, our supplier has told us that the antibody will recognise other syntaxin isoforms in Western Blotting. (please see the discussion in the enquiry submitted on Thursday 24-June-2004). The supplier also advised that the Galli et al reference also features the use of this antibody in immunofluorescence. A suggestion would be to determine the staining pattern you find with the one observed in this publication.

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Answer

This antibody was raised against a fusion protein with the cytoplasmic domain of Syntaxin 3.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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