Overview

  • Product name

    Anti-Syntaxin 3 antibody [EPR8543]
    See all Syntaxin 3 primary antibodies
  • Description

    Rabbit monoclonal [EPR8543] to Syntaxin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human Syntaxin 3 (Q13277).

  • Positive control

    • HepG2, 293T, BxPC-3, Raw 264.7, PC-12, NIH/3T3, Caco-2 cell lysate; Human kidney tissue; HepG2 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-Syntaxin 3 antibody [EPR8543] (ab133750) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : BxPC-3 cell lysate
      Lane 4 : Raw 264.7 cell lysate
      Lane 5 : PC12 cell lysate
      Lane 6 : NIH3T3 cell lysate
      Lane 7 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Syntaxin 3 with ab133750 at 1/50.
    • Immunocytochemical analysis of HepG2 cells labelling Syntaxin 3 with ab133750 at 1/50.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Carta G  et al. Preventive Effects of Resveratrol on Endocannabinoid System and Synaptic Protein Modifications in Rat Cerebral Cortex Challenged by Bilateral Common Carotid Artery Occlusion and Reperfusion. Int J Mol Sci 19:N/A (2018). WB . Read more (PubMed: 29385102) »
    • Mosa MH  et al. Dynamic Formation of Microvillus Inclusions During Enterocyte Differentiation in Munc18-2-Deficient Intestinal Organoids. Cell Mol Gastroenterol Hepatol 6:477-493.e1 (2018). Read more (PubMed: 30364784) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133750.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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