Product nameAnti-Syntaxin 3 antibody [EPR8543]
See all Syntaxin 3 primary antibodies
DescriptionRabbit monoclonal [EPR8543] to Syntaxin 3
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a region within Human Syntaxin 3 (Q13277).
- HepG2, 293T, BxPC-3, Raw 264.7, PC-12, NIH/3T3, Caco-2 cell lysate; Human kidney tissue; HepG2 cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.34 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/50 - 1/100.|
FunctionPotentially involved in docking of synaptic vesicles at presynaptic active zones.
Sequence similaritiesBelongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
- Information by UniProt
- FLJ30906 antibody
- Stx3 antibody
- STX3_HUMAN antibody
All lanes : Anti-Syntaxin 3 antibody [EPR8543] (ab133750) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : BxPC-3 cell lysate
Lane 4 : Raw 264.7 cell lysate
Lane 5 : PC12 cell lysate
Lane 6 : NIH3T3 cell lysate
Lane 7 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Syntaxin 3 with ab133750 at 1/50.
Immunocytochemical analysis of HepG2 cells labelling Syntaxin 3 with ab133750 at 1/50.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Carta G et al. Preventive Effects of Resveratrol on Endocannabinoid System and Synaptic Protein Modifications in Rat Cerebral Cortex Challenged by Bilateral Common Carotid Artery Occlusion and Reperfusion. Int J Mol Sci 19:N/A (2018). WB . Read more (PubMed: 29385102) »
- Mosa MH et al. Dynamic Formation of Microvillus Inclusions During Enterocyte Differentiation in Munc18-2-Deficient Intestinal Organoids. Cell Mol Gastroenterol Hepatol 6:477-493.e1 (2018). Read more (PubMed: 30364784) »