Recombinant Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
![Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)](https://www.abcam.com/ps/products/184/ab184545/Images/ab184545-263405-anti-syntaxin-4-antibody-epr15473-western-blot.jpg "Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15473] to Syntaxin 4
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Syntaxin 4 antibody [EPR15473]
See all Syntaxin 4 primary antibodies -
Description
Rabbit monoclonal [EPR15473] to Syntaxin 4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293, K562, HeLa and A431 cell lysates. Wild-type HAP1 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15473 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones. -
Tissue specificity
Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6810 Human
- Entrez Gene: 20909 Mouse
- Entrez Gene: 81803 Rat
- Omim: 186591 Human
- SwissProt: Q12846 Human
- SwissProt: P70452 Mouse
- SwissProt: Q08850 Rat
- Unigene: 83734 Human
see all -
Alternative names
- NY REN 31 antigen antibody
- p35 2 antibody
- PLACENTAL antibody
see all
Images
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Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Syntaxin 4 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab184545 observed at 1X kDa. Red - loading control, ab18058, observed at 124 kDa.
ab184545 was shown to recognize Syntaxin 4 when Syntaxin 4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Syntaxin 4 knockout samples were subjected to SDS-PAGE. ab184545 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)](https://www.abcam.com/ps/products/184/ab184545/Images/ab184545-216768-anti-syntaxin-4-antibody-epr15473-western-blot.jpg)
Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)All lanes : Anti-Syntaxin 4 antibody [EPR15473] (ab184545) at 1/2000 dilution
Lane 1 : 293 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa -
![Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)](https://www.abcam.com/ps/products/184/ab184545/Images/ab184545-216771-anti-syntaxin-4-antibody-epr15473-western-blot.jpg)
Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)Anti-Syntaxin 4 antibody [EPR15473] (ab184545) at 1/10000 dilution + A431 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa -
![Anti-Syntaxin 4 antibody [EPR15473] (ab184545)](https://www.abcam.com/ps/products/184/ab184545/Images/ab184545-4-benefits-of-recombinant-antibodies.png)
Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (3)
ab184545 has been referenced in 3 publications.
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Lizarbe B et al. Neurochemical Modifications in the Hippocampus, Cortex and Hypothalamus of Mice Exposed to Long-Term High-Fat Diet. Front Neurosci 12:985 (2018). PubMed: 30670942
- Machitani M et al. MicroRNA miR-27 Inhibits Adenovirus Infection by Suppressing the Expression of SNAP25 and TXN2. J Virol 91:N/A (2017). WB ; Human . PubMed: 28356525
