Overview

  • Product name

    Anti-Syntaxin 4 antibody - N-terminal
    See all Syntaxin 4 primary antibodies
  • Description

    Rabbit polyclonal to Syntaxin 4 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human Syntaxin 4 aa 1-50 (N terminal). The exact sequence is proprietary. (NP_004595.2).
    Database link: Q12846

  • Positive control

    • IP: HeLa whole cell lysate.
  • General notes

    ab226218 has not performed satisfactorily when used for WB of Syntaxin 4 in crude preparations (e.g. whole cell lysate).  This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Syntaxin 4.

Properties

Applications

Our Abpromise guarantee covers the use of ab226218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • Syntaxin 4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226218 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-Syntaxin 4 antibody at 1 µg/ml.

      Lane 1: ab226218 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 3 minutes.

    References

    ab226218 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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