Anti-Syntaxin-5 antibody (ab211417)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Syntaxin-5 antibody
See all Syntaxin-5 primary antibodies -
Description
Rabbit polyclonal to Syntaxin-5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human Syntaxin-5 aa 51-307. The exact sequence is proprietary. NCBI Accession No. NP_003155.2.
Sequence:PPDTMSCRDR TQEFLSACKS LQTRQNGIQT NKPALRAVRQ RSEFTLMAKR IGKDLSNTFA KLEKLTILAK RKSLFDDKAV EIEELTYIIK QDINSLNKQI AQLQDFVRAK GSQSGRHLQT HSNTIVVSLQ SKLASMSNDF KSVLEVRTEN LKQQRSRREQ FSRAPVSALP LAPNHLGGGA VVLGAESHAS KDVAIDMMDS RTSQQLQLID EQDSYIQSRA DTMQNIESTI VELGSIFQQL AHMVKEQEET IQRIDEN
Database link: Q13190 -
Positive control
- A431 and rat PC12 whole cell lysates; Human brain tissue.
-
General notes
Previously labelled as Syntaxin 5A.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1.79% Tris glycine -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab211417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| IHC-P | Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
Target
-
Function
Mediates endoplasmic reticulum to Golgi transport. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6811 Human
- Entrez Gene: 65134 Rat
- Omim: 603189 Human
- SwissProt: Q13190 Human
- SwissProt: Q08851 Rat
- Unigene: 654602 Human
- Unigene: 5782 Rat
-
Alternative names
- OTTHUMP00000185028 antibody
- SED5 antibody
- STX5 antibody
see all
Images
-
Western blot - Anti-Syntaxin-5 antibody (ab211417)Anti-Syntaxin-5 antibody (ab211417) at 1/5000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 40 kDa12% SDS PAGE
-
Western blot - Anti-Syntaxin-5 antibody (ab211417)Anti-Syntaxin-5 antibody (ab211417) at 1/5000 dilution + rat PC12 whole cell lysate at 30 µg
Predicted band size: 40 kDa12% SDS PAGE
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin-5 antibody (ab211417)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue, labeling Syntaxin-5 using ab211417 at 7.5 µg/ml.
Protocols
References
ab211417 has not yet been referenced specifically in any publications.
