Anti-Syntaxin-5 antibody (ab211417)
Key features and details
- Rabbit polyclonal to Syntaxin-5
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Syntaxin-5 antibody
See all Syntaxin-5 primary antibodies -
Description
Rabbit polyclonal to Syntaxin-5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment within Human Syntaxin-5 aa 51-307. The exact sequence is proprietary. NCBI Accession No. NP_003155.2.
Sequence:PPDTMSCRDR TQEFLSACKS LQTRQNGIQT NKPALRAVRQ RSEFTLMAKR IGKDLSNTFA KLEKLTILAK RKSLFDDKAV EIEELTYIIK QDINSLNKQI AQLQDFVRAK GSQSGRHLQT HSNTIVVSLQ SKLASMSNDF KSVLEVRTEN LKQQRSRREQ FSRAPVSALP LAPNHLGGGA VVLGAESHAS KDVAIDMMDS RTSQQLQLID EQDSYIQSRA DTMQNIESTI VELGSIFQQL AHMVKEQEET IQRIDEN
Database link: Q13190 -
General notes
Previously labelled as Syntaxin 5A.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1.79% Tris glycine -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab211417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
Target
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Function
Mediates endoplasmic reticulum to Golgi transport. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 6811 Human
- Entrez Gene: 65134 Rat
- Omim: 603189 Human
- SwissProt: Q13190 Human
- SwissProt: Q08851 Rat
- Unigene: 654602 Human
- Unigene: 5782 Rat
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Alternative names
- OTTHUMP00000185028 antibody
- SED5 antibody
- STX5 antibody
see all
Images
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Western blot - Anti-Syntaxin-5 antibody (ab211417)Anti-Syntaxin-5 antibody (ab211417) at 1/5000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 40 kDa12% SDS PAGE
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Western blot - Anti-Syntaxin-5 antibody (ab211417)Anti-Syntaxin-5 antibody (ab211417) at 1/5000 dilution + rat PC12 whole cell lysate at 30 µg
Predicted band size: 40 kDa12% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin-5 antibody (ab211417)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue, labeling Syntaxin-5 using ab211417 at 7.5 µg/ml.
Protocols
References (0)
ab211417 has not yet been referenced specifically in any publications.
