Overview

  • Product name

  • Description

    Rabbit polyclonal to Syntaxin-5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within Human Syntaxin-5 aa 1-50. The exact sequence is proprietary.
    Sequence:

    MIPRKRYGSK NTDQGVYLGL SKTQVLSPAT AGSSSSDIAP LPPPVTLVPP


    Database link: Q13190

  • Positive control

    • IP: HeLa whole cell lysate.
  • General notes

    Previously labelled as Syntaxin 5A. 

     

Properties

Applications

Our Abpromise guarantee covers the use of ab217130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.

ab217130 has not performed satisfactorily when used for WB in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) preparations.

IP Use at 2-10 µg/mg of lysate.

Target

Images

  • Syntaxin-5 was immunoprecipitated from 0.5 to 1mg of HeLa whole cell lysate (prepared using NETN lysis buffer) with ab217130 or control IgG at 6 µg.

    Western blot was performed on the immunoprecipitates (20% of IP loaded) using ab217130 at 1 µg/mL.

    Lane 1: ab127130 used in IP.

    Lane 2: Control IgG used in IP.

     

     

References

ab217130 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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