Key features and details
- Rabbit polyclonal to Syntaxin-5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Syntaxin-5 antibody
See all Syntaxin-5 primary antibodies
DescriptionRabbit polyclonal to Syntaxin-5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- IP: HeLa whole cell lysate.
Previously labelled as Syntaxin 5A.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab217130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
ab217130 has not performed satisfactorily when used for WB in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) preparations.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMediates endoplasmic reticulum to Golgi transport.
Sequence similaritiesBelongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
Cellular localizationEndoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus membrane.
- Information by UniProt
- OTTHUMP00000185028 antibody
- SED5 antibody
- STX5 antibody
Syntaxin-5 was immunoprecipitated from 0.5 to 1mg of HeLa whole cell lysate (prepared using NETN lysis buffer) with ab217130 or control IgG at 6 µg.
Western blot was performed on the immunoprecipitates (20% of IP loaded) using ab217130 at 1 µg/mL.
Lane 1: ab127130 used in IP.
Lane 2: Control IgG used in IP.
ab217130 has been referenced in 1 publication.
- Malik N et al. Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. Biochem J 476:3081-3107 (2019). PubMed: 31665227