Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370)
Key features and details
- Mouse monoclonal [3D10] to Syntaxin 6 - Golgi Membrane Marker
- Suitable for: Flow Cyt, WB, IP
- Reacts with: Mouse, Rat
- Isotype: IgG1
Overview
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Product name
Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker
See all Syntaxin 6 primary antibodies -
Description
Mouse monoclonal [3D10] to Syntaxin 6 - Golgi Membrane Marker -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant full length protein corresponding to Rat Syntaxin 6.
Database link: Q63635 -
Epitope
The epitope for this antibody has been localized to the amino terminus (residues 1-25) of Syntaxin 6. -
Positive control
- Mouse Brain Tissue Extract, rat Brain Tissue Extract.
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General notes
This product was changed from ascites to tissue culture supernatant on 5th July 2019. Lot numbers higher than GR3173630 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
3D10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 30.6 kDa).
|
IP |
Use at an assay dependent concentration.
|
Notes |
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Flow Cyt
Use 2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 30.6 kDa). |
IP
Use at an assay dependent concentration. |
Target
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Function
Involved in intracellular vesicle trafficking. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 58244 Mouse
- Entrez Gene: 60562 Rat
- SwissProt: Q9JKK1 Mouse
- SwissProt: Q63635 Rat
- Unigene: 465911 Mouse
- Unigene: 66264 Mouse
- Unigene: 100217 Rat
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Alternative names
- LAMB2 antibody
- LAMC1 antibody
- Laminin B2 chain antibody
see all
Images
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All lanes : Anti-Syntaxin 6 antibody [3D10] - Golgi Membrane Marker (ab12370) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30.6 kDa
Observed band size: 30.6 kDa
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis image was generated using the ascites version of the product.
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Intracellular membranes from CHO cells were separated on a 10–24% discontinuous Nycodenz gradient. Western Blotting was then performed using a range of antibodies including ab12370 at a 1/1000 dilution. An HRP-conjugated anti-mouse secondary was used at a 1/6000 dilution.
This image was generated using the ascites version of the product.
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Syntaxin 6 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 10µg of Mouse monoclonal to Syntaxin 6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab12370.
Secondary: Protein G-HRP at 1/500 dilution.
Band: 25kDa:Syntaxin 6.This image was generated using the ascites version of the product.
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Overlay histogram showing PC12 cells stained with ab12370 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12370, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab12370 has been referenced in 25 publications.
- Bremner SK et al. Pleiotropic effects of Syntaxin16 identified by gene editing in cultured adipocytes. Front Cell Dev Biol 10:1033501 (2022). PubMed: 36467416
- Brumfield A et al. Insulin-promoted mobilization of GLUT4 from a perinuclear storage site requires RAB10. Mol Biol Cell 32:57-73 (2021). PubMed: 33175605
- Su Q et al. Exosomal LINC00174 derived from vascular endothelial cells attenuates myocardial I/R injury via p53-mediated autophagy and apoptosis. Mol Ther Nucleic Acids 23:1304-1322 (2021). PubMed: 33717651
- Dembla E & Becherer U Biogenesis of large dense core vesicles in mouse chromaffin cells. Traffic 22:78-93 (2021). PubMed: 33369005
- Liao J et al. Upregulation of Piezo1 (Piezo Type Mechanosensitive Ion Channel Component 1) Enhances the Intracellular Free Calcium in Pulmonary Arterial Smooth Muscle Cells From Idiopathic Pulmonary Arterial Hypertension Patients. Hypertension 77:1974-1989 (2021). PubMed: 33813851