Product nameAnti-Syntaxin 6 antibody [3D10]
See all Syntaxin 6 primary antibodies
DescriptionMouse monoclonal [3D10] to Syntaxin 6
Tested applicationsSuitable for: Flow Cyt, WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Hamster, Human
Recombinant rat syntaxin 6 fusion protein.
EpitopeThe epitope for this antibody has been localized to the amino terminus (residues 1-25) of Syntaxin 6.
- Mouse Brain Tissue Extract, rat Brain Tissue Extract.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab12370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30.6 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionInvolved in intracellular vesicle trafficking.
Sequence similaritiesBelongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- LAMB2 antibody
- LAMC1 antibody
- Laminin B2 chain antibody
ab12370 at 1/200 staining a mouse macrophage cell line by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 24 hours. A FITC conjugated donkey anti-mouse antibody was used as the secondary.
All lanes : Anti-Syntaxin 6 antibody [3D10] (ab12370) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30.6 kDa
Observed band size: 30.6 kDa
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Intracellular membranes from CHO cells were separated on a 10–24% discontinuous Nycodenz gradient. Western Blotting was then performed using a range of antibodies including ab12370 at a 1/1000 dilution. An HRP-conjugated anti-mouse secondary was used at a 1/6000 dilution.
Syntaxin 6 was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 10µg of Mouse monoclonal to Syntaxin 6 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab12370.
Secondary: Protein G-HRP at 1/500 dilution.
Band: 25kDa:Syntaxin 6.
Overlay histogram showing PC12 cells stained with ab12370 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12370, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Ceder MM et al. The Neuronal and Peripheral Expressed Membrane-Bound UNC93A Respond to Nutrient Availability in Mice. Front Mol Neurosci 10:351 (2017). Read more (PubMed: 29163028) »
- Sotthibundhu A et al. Rapamycin regulates autophagy and cell adhesion in induced pluripotent stem cells. Stem Cell Res Ther 7:166 (2016). ICC/IF ; Human . Read more (PubMed: 27846905) »