For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    syntaxin-6-antibody-ep7665-golgi-membrane-marker-ab140607.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Protein Trafficking Golgi Proteins
Share by email
RabMAb

Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
  • Western blot - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)

Key features and details

  • Rabbit monoclonal [EP7665] to Syntaxin 6 - Golgi Membrane Marker
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
Product image
Anti-Giantin antibody [9B6] - Golgi Marker (ab37266)
Primary
Product image
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649)

View more associated products

Overview

  • Product name

    Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker
    See all Syntaxin 6 primary antibodies
  • Description

    Rabbit monoclonal [EP7665] to Syntaxin 6 - Golgi Membrane Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human Syntaxin 6 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human Syntaxin 6 protein (ab112390) can be used as a positive control in WB. PC-12 and 293T cell lysate, Human colon and Human kidney tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP7665
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Syntaxin 6 protein (ab171693)
  • Related Products

    • Golgi Staining Kit - Green Fluorescence - Cytopainter (ab139483)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in intracellular vesicle trafficking.
  • Sequence similarities

    Belongs to the syntaxin family.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Golgi apparatus membrane.
  • Target information above from: UniProt accession O43752 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10228 Human
    • Entrez Gene: 58244 Mouse
    • Entrez Gene: 60562 Rat
    • Omim: 603944 Human
    • SwissProt: O43752 Human
    • SwissProt: Q9JKK1 Mouse
    • SwissProt: Q63635 Rat
    • Unigene: 518417 Human
    • Unigene: 465911 Mouse
    • Unigene: 66264 Mouse
    • Unigene: 100217 Rat
    see all
  • Alternative names

    • LAMB2 antibody
    • LAMC1 antibody
    • Laminin B2 chain antibody
    • Laminin-1 subunit gamma antibody
    • Laminin-10 subunit gamma antibody
    • Laminin-11 subunit gamma antibody
    • STX 6 antibody
    • STX6 antibody
    • STX6_HUMAN antibody
    • Syntaxin-6 antibody
    • Syntaxin6 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Syntaxin 6 with ab140607 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
    Western blot - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
    All lanes : Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607) at 1/1000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Syntaxin 6 with ab140607 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (4)

Publishing research using ab140607? Please let us know so that we can cite the reference in this datasheet.

ab140607 has been referenced in 4 publications.

  • Sudhakar JN  et al. Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas. Nat Commun 11:4286 (2020). PubMed: 32855403
  • Hieronimus B  et al. Expression and Characterization of Membrane-Type 4 Matrix Metalloproteinase (MT4-MMP) and its Different Forms in Melanoma. Cell Physiol Biochem 42:198-210 (2017). PubMed: 28531887
  • Seko Y  et al. Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci Rep 5:13737 (2015). PubMed: 26348594
  • Wu X  et al. Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus. J Proteomics 91:136-50 (2013). WB ; Human . PubMed: 23856606

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Fibroblast)
Permeabilization
Yes - 0.25% Triton X-100
Specification
Fibroblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Andrew Schmaus

Verified customer

Submitted Aug 28 2017

Western blot abreview for Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Guinea pig Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10 %)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Monika Schumacher

Verified customer

Submitted Jan 23 2015

Western blot abreview for Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (Membrane Fractions from Sk-Mel-28 human melanoma c)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
0 µg
Specification
Membrane Fractions from Sk-Mel-28 human melanoma c
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Monika Schumacher

Verified customer

Submitted Jan 19 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.