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RabMAb

Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)

Reviews (3) Submit a question References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
  • Western blot - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)

Key features and details

  • Rabbit monoclonal [EP7665] to Syntaxin 6 - Golgi Membrane Marker
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker
    See all Syntaxin 6 primary antibodies

  • Description

    Rabbit monoclonal [EP7665] to Syntaxin 6 - Golgi Membrane Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide within Human Syntaxin 6 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human Syntaxin 6 protein (ab112390) can be used as a positive control in WB. PC-12 and 293T cell lysate, Human colon and Human kidney tissues.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP7665

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Syntaxin 6 with ab140607 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
    Western blot - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
    All lanes : Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607) at 1/1000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Syntaxin 6 with ab140607 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References (4)

Publishing research using ab140607? Please let us know so that we can cite the reference in this datasheet.

ab140607 has been referenced in 4 publications.

  • Sudhakar JN  et al. Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas. Nat Commun 11:4286 (2020). PubMed: 32855403
  • Hieronimus B  et al. Expression and Characterization of Membrane-Type 4 Matrix Metalloproteinase (MT4-MMP) and its Different Forms in Melanoma. Cell Physiol Biochem 42:198-210 (2017). PubMed: 28531887
  • Seko Y  et al. Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci Rep 5:13737 (2015). PubMed: 26348594
  • Wu X  et al. Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus. J Proteomics 91:136-50 (2013). WB ; Human . PubMed: 23856606

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker

 Inconclusive
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Fibroblast)
Permeabilization
Yes - 0.25% Triton X-100
Specification
Fibroblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Andrew Schmaus

Verified customer

Submitted Aug 28 2017

Western blot abreview for Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Guinea pig Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10 %)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Monika Schumacher

Verified customer

Submitted Jan 23 2015

Western blot abreview for Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (Membrane Fractions from Sk-Mel-28 human melanoma c)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
0 µg
Specification
Membrane Fractions from Sk-Mel-28 human melanoma c
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Monika Schumacher

Verified customer

Submitted Jan 19 2015

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