Overview

  • Product name

    Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker
    See all Syntaxin 6 primary antibodies
  • Description

    Rabbit monoclonal [EP7665] to Syntaxin 6 - Golgi Membrane Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Syntaxin 6 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human Syntaxin 6 protein (ab112390) can be used as a positive control in WB. PC-12 and 293T cell lysate, Human colon and Human kidney tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP7665
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.

Target

Images

  • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Syntaxin 6 with ab140607 antibody at a dilution of 1/100.
  • All lanes : Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607) at 1/1000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Syntaxin 6 with ab140607 antibody at a dilution of 1/100.

References

This product has been referenced in:

  • Seko Y  et al. Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci Rep 5:13737 (2015). Read more (PubMed: 26348594) »
  • Wu X  et al. Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus. J Proteomics 91:136-50 (2013). WB ; Human . Read more (PubMed: 23856606) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Fibroblast)
Permeabilization
Yes - 0.25% Triton X-100
Specification
Fibroblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde

Andrew Schmaus

Verified customer

Submitted Aug 28 2017

Application
Western blot
Sample
Guinea pig Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (10 %)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mrs. Monika Schumacher

Verified customer

Submitted Jan 23 2015

Application
Western blot
Sample
Human Cell lysate - other (Membrane Fractions from Sk-Mel-28 human melanoma c)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
0 µg
Specification
Membrane Fractions from Sk-Mel-28 human melanoma c
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mrs. Monika Schumacher

Verified customer

Submitted Jan 19 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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