Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607)
Key features and details
- Rabbit monoclonal [EP7665] to Syntaxin 6 - Golgi Membrane Marker
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker
See all Syntaxin 6 primary antibodies -
Description
Rabbit monoclonal [EP7665] to Syntaxin 6 - Golgi Membrane Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human Syntaxin 6 aa 100-200. The exact sequence is proprietary.
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Positive control
- Recombinant Human Syntaxin 6 protein (ab112390) can be used as a positive control in WB. PC-12 and 293T cell lysate, Human colon and Human kidney tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP7665 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Involved in intracellular vesicle trafficking. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 10228 Human
- Entrez Gene: 58244 Mouse
- Entrez Gene: 60562 Rat
- Omim: 603944 Human
- SwissProt: O43752 Human
- SwissProt: Q9JKK1 Mouse
- SwissProt: Q63635 Rat
- Unigene: 518417 Human
see all -
Alternative names
- LAMB2 antibody
- LAMC1 antibody
- Laminin B2 chain antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Syntaxin 6 with ab140607 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Syntaxin 6 antibody [EP7665] - Golgi Membrane Marker (ab140607) at 1/1000 dilution
Lane 1 : PC-12 cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 29 kDa -
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Syntaxin 6 with ab140607 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab140607 has been referenced in 6 publications.
- Zhang Y et al. Role of STX6 as a prognostic factor associated with immune infiltration in hepatocellular carcinoma. Oncol Lett 24:371 (2022). PubMed: 36238841
- Li W et al. Syntaxin-6, a Reliable Biomarker for Predicting the Prognosis of Patients with Cancer and the Effectiveness of Immunotherapy. Cancers (Basel) 15:N/A (2022). PubMed: 36612024
- Sudhakar JN et al. Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas. Nat Commun 11:4286 (2020). PubMed: 32855403
- Hieronimus B et al. Expression and Characterization of Membrane-Type 4 Matrix Metalloproteinase (MT4-MMP) and its Different Forms in Melanoma. Cell Physiol Biochem 42:198-210 (2017). PubMed: 28531887
- Seko Y et al. Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci Rep 5:13737 (2015). PubMed: 26348594
- Wu X et al. Mitochondrial proteomic analysis of human host cells infected with H3N2 swine influenza virus. J Proteomics 91:136-50 (2013). WB ; Human . PubMed: 23856606