Product nameAnti-Syntaxin 7 antibody
See all Syntaxin 7 primary antibodies
DescriptionRabbit polyclonal to Syntaxin 7
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192923 was affinity purified using an epitope specific to Syntaxin 7 immobilized on solid support.
Our Abpromise guarantee covers the use of ab192923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 29 kDa.|
FunctionMay be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.
Tissue specificityHighest expression is found in placenta followed by heart, skeletal muscle, kidney and brain. Low expression is found in pancreas, lung and liver.
Sequence similaritiesBelongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
Cellular localizationEarly endosome membrane.
- Information by UniProt
- STX7 antibody
- STX7_HUMAN antibody
- Syn7 antibody
- Syntaxin-7 antibody
Detection of Syntaxin 7 by Western Blot of Immunprecipitate.
Lane 1: ab192923 at 1 µg/ml labeling Syntaxin 7 in 293T whole cell lysate immunoprecipitated using ab192923 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-Syntaxin 7 antibody (ab192923) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 30 seconds
Lanes 1-3 prepared in RIPA lysis buffer; Lanes 4-5 prepared in NETN lysis buffer.
ab192923 has not yet been referenced specifically in any publications.