Key features and details
- Mouse monoclonal [CL0257] to Syntaxin 7
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Syntaxin 7 antibody [CL0257]
See all Syntaxin 7 primary antibodies
DescriptionMouse monoclonal [CL0257] to Syntaxin 7
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
- IHC-P: Human melanoma and lymph node tissues. WB: Human tonsil tissue lysate. ICC/IF: U-251 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab211461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 1 µg/ml.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes.
Tissue specificityHighest expression is found in placenta followed by heart, skeletal muscle, kidney and brain. Low expression is found in pancreas, lung and liver.
Sequence similaritiesBelongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain.
Cellular localizationEarly endosome membrane.
- Information by UniProt
- STX7 antibody
- STX7_HUMAN antibody
- Syn7 antibody
- Syntaxin-7 antibody
Immunohistochemical analysis of human lymph node tissue labeling Syntaxin 7 with ab211461 at 1/500 dilution.
Shows strong membrane positivity in non-germinal center cells.
Immunocytochemistry/Immunofluorescence analysis of U-251 cells labelling Syntaxin 7 with ab211461 at 2µg/ml.
Shows specific staining in vesicles in green. Microtubule and nuclear probes are visualized in red and blue, respectively.
Anti-Syntaxin 7 antibody [CL0257] (ab211461) at 1/500 dilution + Human tonsil tissue lysate
Immunohistochemical analysis of human melanoma tissue labeling Syntaxin 7 with ab211461 at 1/500 dilution.
Shows strong cytoplasmic and membrane positivity in tumour cells.
ab211461 has not yet been referenced specifically in any publications.